التفاصيل البيبلوغرافية
| العنوان: |
Bufo marinus bladder H-K-ATPase carries out electroneutral ion transport |
| المؤلفون: |
Burnay, M., Crambert, G., Kharoubi-Hess, S., Geering, K., Horisberger, J. D. |
| المصدر: |
American Journal of Physiology. Renal Physiology, vol. 281, no. 5, pp. F869-74 |
| سنة النشر: |
2001 |
| المجموعة: |
Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: |
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology Animals Bicarbonates/pharmacology Biological Transport *Bufo marinus Carbon Dioxide/pharmacology Electric Conductivity Female Gene Expression H(+)-K(+)-Exchanging ATPase/genetics/*metabolism Hydrogen-Ion Concentration Membrane Potentials Na(+)-K(+)-Exchanging ATPase/metabolism Oocytes/metabolism Rubidium Radioisotopes/metabolism Transfection Urinary Bladder/*enzymology Xenopus laevis |
| الوصف: |
Bufo marinus bladder H-K-ATPase belongs to the Na-K-ATPase and H-K-ATPase subfamily of oligomeric P-type ATPases and is closely related to rat and human nongastric H-K-ATPases. It has been demonstrated that this ATPase transports K(+) into the cell in exchange for protons and sodium ions, but the stoichiometry of this cation exchange is not yet known. We studied the electrogenic properties of B. marinus bladder H-K-ATPase expressed in Xenopus laevis oocytes. In a HEPES-buffered solution, K(+) activation of the H-K-ATPase induced a slow-onset inward current that reached an amplitude of approximately 20 nA after 1-2 min. When measurements were performed in a solution containing 25 mM HCO at a PCO(2) of 40 Torr, the negative current activated by K(+) was reduced. In noninjected oocytes, intracellular alkalization activated an inward current similar to that due to B. marinus H-K-ATPase. We conclude that the transport activity of the nongastric B. marinus H-K-ATPase is not intrinsically electrogenic but that the inward current observed in oocytes expressing this ion pump is secondary to intracellular alkalization induced by proton transport. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
info:eu-repo/semantics/altIdentifier/pmid/11592945; info:eu-repo/semantics/altIdentifier/pissn/0363-6127; https://serval.unil.ch/notice/serval:BIB_DAAF2C60C5E0Test |
| الإتاحة: |
https://serval.unil.ch/notice/serval:BIB_DAAF2C60C5E0Test |
| رقم الانضمام: |
edsbas.B80DB017 |
| قاعدة البيانات: |
BASE |
