دورية أكاديمية
Splicing of Cauliflower mosaic virus 35S RNA serves to downregulate a toxic gene product
| العنوان: | Splicing of Cauliflower mosaic virus 35S RNA serves to downregulate a toxic gene product |
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| المؤلفون: | Froissart, Rémy, Uzest-Bonhomme, Marilyne, Ruiz-Ferrer, Virginia, Drucker, Martin, Hébrard, Eugénie, Hohn, Thomas, Blanc, Stéphane |
| المساهمون: | UMR INRA / ENSAM / CIRAD : Biologie et Génétique des Interactions Plantes / Parasite pour la Protection Intégrée, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Montpellier (ENSA M), Diversité et génomes des plantes cultivées (UMR DGPC), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Friedrich Miescher Institute for Biomedical Research |
| المصدر: | ISSN: 0022-1317. |
| بيانات النشر: | HAL CCSD Microbiology Society |
| سنة النشر: | 2004 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | PLANT PATHOGEN, CAULIFLOWER MOSAIC VIRUS, CAULIMOVIRIDAE, VIROLOGIE, BIOLOGIE DES POPULATIONS, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| الوصف: | International audience ; Alternative splicing usually leads to an increase in the number of gene products that can be derived from a single transcript. Here, a different and novel use of alternative splicing - as a means to control the amount of a potentially toxic gene product in the plant pararetrovirus Cauliflower mosaic virus (CaMV) - is reported. About 70 % of the CaMV 35S RNA, which serves as a substrate for both reverse transcription and polycistronic mRNA, is spliced into four additional RNA species. Splicing occurs between four donor sites - one in the 5' untranslated region and three within open reading frame (ORF) I - and one unique acceptor site at position 1508 in ORF II. A previous study revealed that the acceptor site is vital for CaMV infectivity and expression of ORFs III and IV from one of the spliced RNA species suggested that splicing may facilitate expression of downstream CaMV ORFs. However, it is shown here that deleting the splice acceptor site and replacing ORF II with a cargo ORF that lacks splice acceptor sites does not interfere with virus proliferation. Furthermore, it is demonstrated that whenever P2 cannot accumulate in infected tissues, the splice acceptor site at position 1508 is no longer vital and has little effect on virus replication. This suggests that the vital role of splicing in CaMV is regulation of P2 expression and that P2 exhibits biological properties that, whilst indispensable for virus-vector interactions, can block in planta virus infection if this regulation is abolished. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02683349; https://hal.inrae.fr/hal-02683349Test; PRODINRA: 14105; WOS: 000223575500023 |
| DOI: | 10.1099/vir.0.80029-0 |
| الإتاحة: | https://doi.org/10.1099/vir.0.80029-0Test https://hal.inrae.fr/hal-02683349Test |
| رقم الانضمام: | edsbas.B7DDC811 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1099/vir.0.80029-0 |
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