دورية أكاديمية
The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary
| العنوان: | The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary |
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| المؤلفون: | Tsunemine, Satoru, Nakagawa, Hiromi, Suzuki, Yutaka, Murakami, Yota |
| المساهمون: | 常峰, 悟, 中川, 浩実 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2022 |
| المجموعة: | Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: | Gene Regulation, Chromatin and Epigenetics |
| الوصف: | Centromeres of most eukaryotes consist of two distinct chromatin domains: a kinetochore domain, identified by the histone H3 variant, CENP-A, and a heterochromatic domain. How these two domains are separated is unclear. Here, we show that, in Schizosaccharomyces pombe, mutation of the chromatin remodeler RSC induced CENP-ACnp1 misloading at pericentromeric heterochromatin, resulting in the mis-assembly of kinetochore proteins and a defect in chromosome segregation. We find that RSC functions at the kinetochore boundary to prevent CENP-ACnp1 from spreading into neighbouring heterochromatin, where deacetylated histones provide an ideal environment for the spread of CENP-ACnp1. In addition, we show that RSC decompacts the chromatin structure at this boundary, and propose that this RSC-directed chromatin decompaction prevents mis-propagation of CENP-ACnp1 into pericentromeric heterochromatin. Our study provides an insight into how the distribution of distinct chromatin domains is established and maintained. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0305-1048 1362-4962 |
| العلاقة: | http://hdl.handle.net/2433/286138Test; Nucleic Acids Research; 50; 19; 10914; 10928 |
| الإتاحة: | http://hdl.handle.net/2433/286138Test |
| حقوق: | © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. ; This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. ; https://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: | edsbas.B7278A6E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 03051048 13624962 |
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