دورية أكاديمية
CD44 modulates cell migration and invasion in Ewing sarcoma cells
| العنوان: | CD44 modulates cell migration and invasion in Ewing sarcoma cells |
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| المؤلفون: | Fernández-Tabanera, Enrique, García-García, Laura, Rodríguez-Martín, Carlos, Cervera, Saint T., González-González, Laura, Robledo, Cristina, Josa, Santiago, Chapado, Luis, Monzón, Sara, Martinez, Selene, Melero Fernandez de Mera, Raquel Maria, Alonso, Javier |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Digital Deposit e-UCJC (Universidad Camilo José Cela) |
| مصطلحات موضوعية: | Biología Celular y Molecular, Ciencias Biomédicas, Ewing sarcoma, CD44, EWSR1::FLI1, cell migration, cell invasiveness |
| الوصف: | The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells. ; : This project was funded by Instituto de Salud Carlos III, grant numbers PI20CIII/00020, DTS18CIII/00005, Asociación Pablo Ugarte, grant numbers TRPV205/18; Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, grant numbers TVP333-19, TVP-1324/15; ASION, grant number TVP141/17. Enrique Fernández-Tabanera is supported by Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, Saint T. Cervera is supported by Asociación Pablo Ugarte and Raquel M. Melero is supported by a CIBERER contract. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 14220067 |
| العلاقة: | Fernández-Tabanera E, García-García L, Rodríguez-Martín C, Cervera ST, González-González L, Robledo C, Josa S, Martínez S, Chapado L, Monzón S, Melero-Fernández de Mera RM, Alonso J. CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells. Int J Mol Sci. 2023 Jul 21;24(14):11774. doi:10.3390/ijms241411774. PMID: 37511533; PMCID: PMC10381016.; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381016Test/; http://hdl.handle.net/20.500.12020/1207Test |
| DOI: | 10.3390/ijms241411774 |
| الإتاحة: | https://doi.org/20.500.12020/1207Test https://doi.org/10.3390/ijms241411774Test https://hdl.handle.net/20.500.12020/1207Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381016Test/ |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; openAccess |
| رقم الانضمام: | edsbas.B6CF5E3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms241411774 |