دورية أكاديمية
A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds
| العنوان: | A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds |
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| المؤلفون: | Tomasina, Clarissa, Mohren, Ronny, Camarero-Espinosa, Sandra, Cillero-Pastor, Berta, Moroni, Lorenzo |
| المصدر: | Tomasina , C , Mohren , R , Camarero-Espinosa , S , Cillero-Pastor , B & Moroni , L 2024 , ' A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds ' , Small Science . https://doi.org/10.1002/smsc.202300316Test |
| سنة النشر: | 2024 |
| المجموعة: | Maastricht University Research Publications |
| مصطلحات موضوعية: | additive manufacturing, bone, cartilage, mesenchymal stem cells, proteomics, ARTICULAR-CARTILAGE, CHONDROGENIC DIFFERENTIATION, OSTEOGENIC DIFFERENTIATION, ENDOCHONDRAL OSSIFICATION, MECHANICAL-PROPERTIES, NEGATIVE REGULATOR, BINDING, EXPRESSION, COLLAGEN, DEGRADATION |
| الوصف: | Understanding how porous biomaterials interact with cells at their surface and how they either promote or inhibit cellular processes has presented several challenges. Additive manufacturing enables the fabrication of scaffolds with distinct compositions and designs for different tissue engineering applications. To evaluate the in vitro performance of multiple printed materials, biochemical assays can be limiting in providing valuable insight and key information to select the best tissue destination. Omics technologies like proteomics are crucial for studying important cellular events and gathering valuable information about cellular processes and mechanisms. However, only few studies focus on proteomics to decipher cell-material interactions and cell differentiation on additive manufactured scaffolds. Here, scaffolds were fabricated using three polymers (polycaprolactone (PCL), poly(ethylene oxide)-poly(butylene terephthalate) (PEOT/PBT), and polylactic acid (PLA)) through additive manufacturing. Their chondrogenic and osteogenic potential were characterized and compared using human bone marrow-derived mesenchymal stem cells (hBMSCs) through proteomics analysis. The 3D scaffolds were all hydrophilic and displayed Young's moduli close to those of bone or cartilage for PLA and PCL and PEOT/PBT, respectively. Biochemical assays indicated that PEOT/PBT and PLA scaffolds have a greater chondrogenic potential by higher glycosaminoglycan (GAG) and collagen deposition compared to PCL. PLA and PEOT/PBT showed to be more effective in promoting bone formation, as evidenced by higher calcium deposits detected by alizarin red staining, and higher alkaline phosphatase (ALP), especially for PLA in osteogenic medium. Proteomics data revealed the most distinct separation between conditions in chondrogenic medium, which had the highest protein identification rates. Pathway analysis showed that PCL did not induce any differentiation-related pathways when compared to PEOT/PBT and PLA in any of the tested media conditions. Analysis ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://cris.maastrichtuniversity.nl/en/publications/1daeb50d-3343-4ca5-ba85-c07051a83b03Test |
| DOI: | 10.1002/smsc.202300316 |
| الإتاحة: | https://doi.org/10.1002/smsc.202300316Test https://cris.maastrichtuniversity.nl/en/publications/1daeb50d-3343-4ca5-ba85-c07051a83b03Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B626C616 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/smsc.202300316 |
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