دورية أكاديمية
Production of Recombinant Active Human TGFβ1 in Nicotiana benthamiana ; Production of Recombinant Active Human TGF beta 1 in Nicotiana benthamiana
| العنوان: | Production of Recombinant Active Human TGFβ1 in Nicotiana benthamiana ; Production of Recombinant Active Human TGF beta 1 in Nicotiana benthamiana |
|---|---|
| المؤلفون: | Soni, Aditya Prakash, Lee, Juhee, Shin, Kunyoo, Koiwa, Hisashi, Hwang, Inhwan |
| المساهمون: | Shin, Kunyoo |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2022 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | GROWTH-FACTOR-BETA, TGF-BETA, PROTEIN EXPRESSION, LATENT, PLANTS, SECRETION, LEVEL, GLYCOSYLATION, GLYCOPROTEIN, PURIFICATION, recombinant proteins, human growth factors, LAP-TGF beta 1, human TGF beta 1, Nicotiana benthamiana, CBM3, acid activation |
| الوصف: | The production of recombinant proteins in plant systems is receiving wider attention. Indeed, various plant-produced pharmaceuticals have been shown to be biologically active. However, the production of human growth factors and cytokines in heterologous systems is still challenging because they often act as complex forms, such as homo- or heterodimers, and their production is tightly regulated in vivo. In this study, we demonstrated that the mature form of human TGF beta 1 produced and purified from Nicotiana benthamiana shows biological activity in animal cells. To produce the mature form of TGF beta 1, various recombinant genes containing the mature form of TGF beta 1 were generated and produced in N. benthamiana. Of these, a recombinant construct, BiP:M:CBM3:LAP[C33S]:EK:TGF beta 1, was expressed at a high level in N. benthamiana. Recombinant proteins were one-step purified using cellulose-binding module 3 (CBM3) as an affinity tag and microcrystalline cellulose (MCC) beads as a matrix. The TGF beta 1 recombinant protein bound on MCC beads was proteolytically processed with enterokinase to separate mature TGF beta 1. The mature TGF beta 1 still associated with Latency Associated Protein, [LAP(C33S)] that had been immobilized on MCC beads was released by HCl treatment. Purified TGF beta 1 activated TGF beta 1-mediated signaling in the A549 cell line, thereby inducing phosphorylation of SMAD-2, the expression of ZEB-2 and SNAIL1, and the formation of a filopodia-like structure. Based on these results, we propose that active mature TGF beta 1, one of the most challenging growth factors to produce in heterologous systems, can be produced from plants at a high degree of purity via a few steps. ; Y ; 1 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1664-462X |
| العلاقة: | Frontiers in Plant Science, Vol.13, p. 922694; https://hdl.handle.net/10371/185147Test; 000811280700001; 2-s2.0-85132314136; 165452 |
| DOI: | 10.3389/fpls.2022.922694 |
| الإتاحة: | https://doi.org/10.3389/fpls.2022.922694Test https://hdl.handle.net/10371/185147Test |
| رقم الانضمام: | edsbas.B5ED0996 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664462X |
|---|---|
| DOI: | 10.3389/fpls.2022.922694 |