دورية أكاديمية
Periodontitis associates with species-specific gene expression of the oral microbiota
| العنوان: | Periodontitis associates with species-specific gene expression of the oral microbiota |
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| المؤلفون: | Belstrøm, Daniel, Constancias, Florentin, Drautz-Moses, Daniela, I, Schuster, Stephan, C, Veleba, Mark, Mahé, Frédéric, Givskov, Michael |
| المساهمون: | University of Copenhagen = Københavns Universitet (UCPH), Singapore Centre for Environmental Life Sciences Engineering Singapore (SCELSE), Nanyang Technological University Singapour, Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), SCELSE |
| المصدر: | EISSN: 2055-5008 ; npj Biofilms and Microbiomes ; https://hal.inrae.fr/hal-03469662Test ; npj Biofilms and Microbiomes, 2021, 7 (1), ⟨10.1038/s41522-021-00247-y⟩ |
| بيانات النشر: | HAL CCSD Springer Nature published in partnership with Nanyang Technological University |
| سنة النشر: | 2021 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; Abstract The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial samples were collected from three oral sites—subgingival plaque, tongue, and saliva from patients with periodontitis and healthy controls. Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. While oral site was the main determinant of taxonomic composition as well as bacterial gene expression, periodontitis was significantly associated with a reduction of carbohydrate metabolism of the oral microbiota at three oral sites (subgingival plaque, tongue, and saliva). Data from the present study revealed the association of periodontitis with bacterial gene expression of the oral microbiota. Conditions of periodontitis was associated with bacterial activity of local subgingival plaque, but also on tongue and the salivary microbiota. Collectively, data suggest that periodontitis associates with impaired carbohydrate metabolism of the oral microbiota. Future longitudinal and interventional studies are warranted to evaluate the potential pathogenic role of impaired bacterial carbohydrate metabolism not only in periodontitis but also in other diseases with low-grade inflammation, such as type 2 diabetes mellitus. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03469662; https://hal.inrae.fr/hal-03469662Test; https://hal.inrae.fr/hal-03469662/documentTest; https://hal.inrae.fr/hal-03469662/file/Belstrom-NPJBM-2021-CC-BY.pdfTest; WOS: 000700278600001 |
| DOI: | 10.1038/s41522-021-00247-y |
| الإتاحة: | https://doi.org/10.1038/s41522-021-00247-yTest https://hal.inrae.fr/hal-03469662Test https://hal.inrae.fr/hal-03469662/documentTest https://hal.inrae.fr/hal-03469662/file/Belstrom-NPJBM-2021-CC-BY.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B59D7468 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41522-021-00247-y |
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