دورية أكاديمية
Nogo-A is secreted in extracellular vesicles, occurs in blood and can influence vascular permeability
| العنوان: | Nogo-A is secreted in extracellular vesicles, occurs in blood and can influence vascular permeability |
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| المؤلفون: | Rust, Ruslan, Holm, Mea M, Egger, Matteo, Weinmann, Oliver, van Rossum, Daniёlle, Walter, Fruzsina R, Santa-Maria, Ana Raquel, Grönnert, Lisa, Maurer, Michael A, Kraler, Simon, Akhmedov, Alexander, Cideciyan, Rose, Lüscher, Thomas F, Deli, Maria A, Herrmann, Inge K, Schwab, Martin E |
| المصدر: | Rust, Ruslan; Holm, Mea M; Egger, Matteo; Weinmann, Oliver; van Rossum, Daniёlle; Walter, Fruzsina R; Santa-Maria, Ana Raquel; Grönnert, Lisa; Maurer, Michael A; Kraler, Simon; Akhmedov, Alexander; Cideciyan, Rose; Lüscher, Thomas F; Deli, Maria A; Herrmann, Inge K; Schwab, Martin E (2024). Nogo-A is secreted in extracellular vesicles, occurs in blood and can influence vascular permeability. Journal of Cerebral Blood Flow and Metabolism, 44(6):938-954. |
| بيانات النشر: | Sage Publications |
| سنة النشر: | 2024 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Institute for Regenerative Medicine (IREM), 610 Medicine & health |
| الوصف: | Nogo-A is a transmembrane protein with multiple functions in the central nervous system (CNS), including restriction of neurite growth and synaptic plasticity. Thus far, Nogo-A has been predominantly considered a cell contact-dependent ligand signaling via cell surface receptors. Here, we show that Nogo-A can be secreted by cultured cells of neuronal and glial origin in association with extracellular vesicles (EVs). Neuron- and oligodendrocyte-derived Nogo-A containing EVs inhibited fibroblast spreading, and this effect was partially reversed by Nogo-A receptor S1PR2 blockage. EVs purified from HEK cells only inhibited fibroblast spreading upon Nogo-A over-expression. Nogo-A-containing EVs were found in vivo in the blood of healthy mice and rats, as well as in human plasma. Blood Nogo-A concentrations were elevated after acute stroke lesions in mice and rats. Nogo-A active peptides decreased barrier integrity in an in vitro blood-brain barrier model. Stroked mice showed increased dye permeability in peripheral organs when tested 2 weeks after injury. In the Miles assay, an in vivo test to assess leakage of the skin vasculature, a Nogo-A active peptide increased dye permeability. These findings suggest that blood borne, possibly EV-associated Nogo-A could exert long-range regulatory actions on vascular permeability. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0271-678X |
| العلاقة: | https://www.zora.uzh.ch/id/eprint/239712/1/ZORA239712.pdfTest; info:pmid/38000040; urn:issn:0271-678X |
| DOI: | 10.5167/uzh-239712 |
| DOI: | 10.1177/0271678X231216270 |
| الإتاحة: | https://doi.org/10.5167/uzh-23971210.1177/0271678X231216270Test https://www.zora.uzh.ch/id/eprint/239712Test/ https://www.zora.uzh.ch/id/eprint/239712/1/ZORA239712.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) ; http://creativecommons.org/licenses/by-nc/4.0Test/ |
| رقم الانضمام: | edsbas.B4BFB508 |
| قاعدة البيانات: | BASE |
| تدمد: | 0271678X |
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| DOI: | 10.5167/uzh-239712 |