دورية أكاديمية
Immunohistochemical evaluation of aquaporin-4 and its correlation with CD68, IBA-1, HIF-1α, GFAP, and CD15 expressions in fatal traumatic brain injury
| العنوان: | Immunohistochemical evaluation of aquaporin-4 and its correlation with CD68, IBA-1, HIF-1α, GFAP, and CD15 expressions in fatal traumatic brain injury |
|---|---|
| المؤلفون: | Neri M., Frati A., Turillazzi E., Cantatore S., Cipolloni L., Di Paolo M., Frati P., La Russa R., Maiese A., Scopetti M., Santurro A., Sessa F., Zamparese R., Fineschi V. |
| المساهمون: | Neri, M., Frati, A., Turillazzi, E., Cantatore, S., Cipolloni, L., Di Paolo, M., Frati, P., La Russa, R., Maiese, A., Scopetti, M., Santurro, A., Sessa, F., Zamparese, R., Fineschi, V. |
| سنة النشر: | 2018 |
| المجموعة: | ARPI - Archivio della Ricerca dell'Università di Pisa |
| مصطلحات موضوعية: | Aquaporin, Computed tomography (CT) scan, Edema, Genetic analysi, Hypoxia, Microglia activation, Traumatic brain injury, Adult, Aged, Antigens, CD, Differentiation, Myelomonocytic, Aquaporin 4, Base Sequence, Brain Edema, Brain Injuries, Traumatic, Calcium-Binding Protein, DNA-Binding Protein, Female, Glial Fibrillary Acidic Protein, Human, Hypoxia-Inducible Factor 1, alpha Subunit, Immunohistochemistry, Lewis X Antigen, Male, Microfilament Protein, Middle Aged |
| الوصف: | Traumatic brain injury (TBI) is one of the leading causes of death and disability worldwide. Our understanding of its pathobiology has substantially increased. Following TBI, the following occur, edema formation, brain swelling, increased intracranial pressure, changes in cerebral blood flow, hypoxia, neuroinflammation, oxidative stress, excitotoxicity, and apoptosis. Experimental animal models have been developed. However, the difficulty in mimicking human TBI explains why few neuroprotective strategies, drawn up on the basis of experimental studies, have translated into improved therapeutic strategies for TBI patients. In this study, we retrospectively examined brain samples in 145 cases of death after different survival times following TBI, to investigate aquaporin-4 (AQP4) expression and correlation with hypoxia, and neuroinflammation in human TBI. Antibodies anti-glial fibrillary acid protein (GFAP), aquaporin-4 (AQP4), hypoxia induced factor-1α (HIF-1α), macrophage/phagocytic activation (CD68), ionized calcium-binding adapter molecule-1 (IBA-1), and neutrophils (CD15) were used. AQP4 showed a significant, progressive increase between the control group and groups 2 (one-day survival) and 3 (three-day survival). There were further increases in AQP4 immunopositivity in groups 4 (seven-day survival), 5 (14-dayssurvival), and 6 (30-day survival), suggesting an upregulation of AQP4 at 7 to 30 days compared to group 1. GFAP showed its highest expression in non-acute cases at the astrocytic level compared with the acute TBI group. Data emerging from the HIF-1α reaction showed a progressive, significant increase. Immunohistochemistry with IBA-1 revealed activated microglia starting three days after trauma and progressively increasing in the next 15 to 20 days after the initial trauma. CD68 expression demonstrated basal macrophage and phagocytic activation mostly around blood vessels. Starting from one to three days of survival after TBI, an increase in the number of CD68 cells was progressively observed; at 15 and ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/30423808; info:eu-repo/semantics/altIdentifier/wos/WOS:000451528500260; volume:19; issue:11; firstpage:3544; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; http://hdl.handle.net/11568/1067007Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85056543279 |
| DOI: | 10.3390/ijms19113544 |
| الإتاحة: | https://doi.org/10.3390/ijms19113544Test http://hdl.handle.net/11568/1067007Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B3A56E32 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms19113544 |
|---|