كتاب
Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
| العنوان: | Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes |
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| المؤلفون: | Vivaudou, Michel, Todorov, Zlatomir, Reyes-Mejia, Gina Catalina, Moreau, Christophe J |
| المساهمون: | Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ANR-11-RPIB-0022,VenomPicoScreen,Découverte de principes actifs à partir de venins en utilisant des bicouches artificielles miniaturisées(2011), ANR-11-LABX-0015,ICST,Canaux ioniques d'intérêt thérapeutique(2011), European Project: NIH |
| المصدر: | Membrane Protein Structure and Function Characterization: Methods and Protocols, Methods in Molecular Biology ; https://hal.science/hal-02007728Test ; Membrane Protein Structure and Function Characterization: Methods and Protocols, Methods in Molecular Biology, 1635, Jean-Jacques Lacapere, pp.283-301, 2017, ⟨10.1007/978-1-4939-7151-0_15⟩ |
| بيانات النشر: | HAL CCSD Jean-Jacques Lacapere |
| سنة النشر: | 2017 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | G-protein-coupled receptor, Ion-channel-coupled receptor, Potassium channel, Robot, Two-electrode voltage clamp, Xenopus oocyte, MESH: Animals, MESH: Automation, Laboratory, MESH: Xenopus Proteins, MESH: Xenopus laevis, MESH: Cell Membrane, MESH: Electrophysiological Phenomena, MESH: Female, MESH: G Protein-Coupled Inwardly-Rectifying Potassium Channels, MESH: Oocytes, MESH: Receptors, G-Protein-Coupled, MESH: Signal Transduction, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] |
| الوصف: | International audience ; G-protein-coupled receptors (GPCR) are the most widely used system of communication used by cells. They sense external signals and translate them into intracellular signals. The information is carried mechanically across the cell membrane, without perturbing its integrity. Agonist binding on the extracellular side causes a change in receptor conformation which propagates to the intracellular side and causes release of activated G-proteins, the first messengers of a variety of signaling cascades.Permitting access to powerful electrophysiological techniques, ion channels can be employed to monitor precisely the most proximal steps of GPCR signaling, receptor conformational changes, and G-protein release. The former is achieved by physical attachment of a potassium channel to the GPCR to create an Ion-Channel Coupled Receptor (ICCR). The latter is based on the use of G-protein-regulated potassium channels (GIRK). We describe here how these two systems may be used in the Xenopus oocyte heterologous system with a robotic system for increased throughput. |
| نوع الوثيقة: | book part |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28755375; hal-02007728; https://hal.science/hal-02007728Test; PUBMED: 28755375 |
| DOI: | 10.1007/978-1-4939-7151-0_15 |
| الإتاحة: | https://doi.org/10.1007/978-1-4939-7151-0_15Test https://hal.science/hal-02007728Test |
| رقم الانضمام: | edsbas.B2324987 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-4939-7151-0_15 |
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