دورية أكاديمية
毛细管电泳法糖化血红蛋白检测系统在缺铁性贫血患者中的应用 ; Capillary electrophoresis for glycated hemoglobin A1c determination in patients with iron deficiency anemia
| العنوان: | 毛细管电泳法糖化血红蛋白检测系统在缺铁性贫血患者中的应用 ; Capillary electrophoresis for glycated hemoglobin A1c determination in patients with iron deficiency anemia |
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| المؤلفون: | 徐安平, 纪玲, 陈卫东, 夏勇, 喻晶, 周宇, 李璐 |
| المساهمون: | 北京大学深圳医院检验科,北京,100000 |
| المصدر: | 知网 ; 万方 ; http://d.g.wanfangdata.com.cn/Periodical_shyxjyzz201604015.aspxTest |
| بيانات النشر: | 检验医学 |
| سنة النشر: | 2016 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | 糖化血红蛋白, 血红蛋白 A2, 毛细管电泳法, 缺铁性贫血, Glycated hemoglobin A1c, Hemoglobin A2, Capillary electrophoresis, Iron deficiency anemia |
| الوصف: | 目的：探讨缺铁性贫血（IDA）对糖化血红蛋白（HbA1c）检测的影响以及毛细管电泳法检测 HbA2在 IDA 中的应用。方法收集 IDA 确诊患者79例，以79名血常规和铁蛋白均正常者作为对照组。分别检测2个组的空腹血糖、糖化血清蛋白、铁蛋白、HbA1c 及 HbA2。采用2种方案研究 IDA 对 HbA1c 检测的影响：（1）检测对照组和 IDA 组 HbA1c，分析 IDA 对 HbA1c 结果的影响；（2）采用毛细管电泳法检测 HbA2，比较IDA 患者治疗前后各项血液学指标[包括血红蛋白（Hb）、红细胞平均体积（MCV）、平均红细胞血红蛋白含量（MCH）]及铁蛋白、HbA1c、HbA2的差异。分析毛细管电泳法检测对照组和 IDA 组的 HbA2结果，通过受试者工作特征（ROC）曲线建立毛细管电泳法 HbA2筛查 IDA 的临界值。结果对照组和 IDA 组空腹血糖和糖化血清蛋白差异均无统计学意义（P 均>0.05），而铁蛋白和 HbA1c 结果差异均有统计学意义（P<0.05）。IDA 组治疗前后 Hb、MCV、MCH、铁蛋白、HbA1c 及 HbA2结果差异均有统计学意义（P<0.01）。毛细管电泳法 HbA2筛查 IDA 的临界值为2.25%，敏感性为93.4%，特异性为92.1%。结论 IDA 对 HbA1c 的检测会产生一定的影响。毛细管电泳法在检测 HbA1c 的同时还能提供 HbA2结果，可用于筛查 IDA。 ; Objective To investigate the influence of iron deficiency anemia(IDA)on glycated hemoglobin A1c(HbA1c)determination and the application of capillary electrophoresis in the determination of hemoglobin A2 (HbA2)for the diagnosis of IDA. Methods A total of 79 patients with IDA were enrolled,and 79 subjects with normal blood routine test results and ferritin were enrolled as control group. Fasting blood glucose,glycated serum protein,ferritin,HbA1c and HbA2 were determined. Using 2 plans,the influence of IDA on HbA1c determination was evaluated.(1)The influence of IDA on the results of HbA1c determination was evaluated.(2)HbA2 was determined by capillary electrophoresis,and the differences of hemoglobin(Hb),mean corpuscular volume(MCV),mean corpuscular hemoglobin(MCH),ferritin,HbA1c and HbA2 before and after treatment were compared. The HbA2 results in control and IDA groups by capillary electrophoresis were analyzed. The cut-off values of HbA2 by capillary electrophoresis for IDA were assessed by receiver operating characteristic(ROC)curve. Results Fasting blood glucose and glycated serum protein showed no statistical significance between control and IDA groups(P>0.05). Ferritin and HbA1c showed statistical significance between control and IDA groups(P<0.05). In IDA group,Hb, MCV,MCH,ferritin,HbA1c and HbA2 had statistical significance between before and after treatment(P<0.01). When ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 1673-8640 |
| العلاقة: | 检验医学.2016,31,(4),299-303.; 1404433; http://hdl.handle.net/20.500.11897/441341Test |
| DOI: | 10.3969/j.issn.1673-8640.2016.04.012 |
| الإتاحة: | https://doi.org/20.500.11897/441341Test https://doi.org/10.3969/j.issn.1673-8640.2016.04.012Test https://hdl.handle.net/20.500.11897/441341Test |
| رقم الانضمام: | edsbas.B060CA85 |
| قاعدة البيانات: | BASE |
| تدمد: | 16738640 |
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| DOI: | 10.3969/j.issn.1673-8640.2016.04.012 |