دورية أكاديمية
Lipopolysaccharide-induced carotid body inflammation in cats: functional manifestations, histopathology and involvement of tumour necrosis factor-alpha
| العنوان: | Lipopolysaccharide-induced carotid body inflammation in cats: functional manifestations, histopathology and involvement of tumour necrosis factor-alpha |
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| المؤلفون: | Fernandez, Ricardo, Gonzalez, Sergio, Rey, Sergio, Cortes, Paula P., Maisey, Kevin R., Reyes, Edison Pablo, Larrain, Carolina, Zapata, Patricio |
| بيانات النشر: | WILEY-BLACKWELL |
| سنة النشر: | 2024 |
| المجموعة: | Pontificia Universidad Católica de Chile: Repositorio UC |
| مصطلحات موضوعية: | DORSAL VAGAL COMPLEX, C-FOS EXPRESSION, RECEPTOR-TYPE-I, SUBDIAPHRAGMATIC VAGOTOMY, ENDOTOXIN INFUSION, ESCHERICHIA-COLI, GLOMUS CELLS, VAGUS NERVE, INTERLEUKIN-1, RAT, 03 Good Health and Well-being, 03 Salud y bienestar |
| الوصف: | In the absence of information on functional manifestations of carotid body (CB) inflammation, we studied an experimental model in which lipopolysaccharide (LPS) administration to pentobarbitone-anaesthetized cats was performed by topical application upon the CB surface or by intravenous infusion (endotoxaemia). The latter caused: (i) disorganization of CB glomoids, increased connective tissue, and rapid recruitment of polymorphonuclear cells into the vascular bed and parenchyma within 4 h; (ii) increased respiratory frequency and diminished ventilatory chemoreflex responses to brief hypoxia (breathing 100% N-2 for 10 s) and diminished ventilatory chemosensory drive (assessed by 100% O-2 tests) during normoxia and hypoxia; (iii) tachycardia, increased haematocrit and systemic hypotension in response to LPS I.V.; and (iv) increased basal frequency of carotid chemosensory discharges during normoxia, but no change in maximal chemoreceptor responses to brief hypoxic exposures. Lipopolysaccharide-induced tachypnoea was prevented by prior bilateral carotid neurotomy. Apoptosis was not observed in CBs from cats subjected to endotoxaemia. Searching for pro-inflammatory mediators, tumour necrosis factor-alpha (TNF-alpha) was localized by immunohistochemistry in glomus and endothelial cells; reverse transcriptase-polymerase chain reaction revealed that the CB expresses the mRNAs for both type-1 (TNF-R1) and type-2 TNF-alpha receptors (TNF-R2); Western blot confirmed a band of the size expected for TNF-R1; and histochemistry showed the presence of TNF-R1 in glomus cells and of TNF-R2 in endothelial cells. Experiments in vitro showed that the frequency of carotid nerve discharges recorded from CBs perfused and superfused under normoxic conditions was not significantly modified by TNF-alpha, but that the enhanced frequency of chemosensory discharges recorded along responses to hypoxic stimulation was transiently diminished in a dose-dependent manner by TNF-alpha injections. The results suggest that the CB may operate as a ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 16 páginas |
| اللغة: | English |
| تدمد: | 1469-445X 0958-0670 |
| العلاقة: | MEDLINE:18562477; https://doi.org/10.1113/expphysiol.2008.041152Test; https://repositorio.uc.cl/handle/11534/79499Test; WOS:000256722300012 |
| DOI: | 10.1113/expphysiol.2008.041152 |
| الإتاحة: | https://doi.org/10.1113/expphysiol.2008.041152Test https://repositorio.uc.cl/handle/11534/79499Test |
| حقوق: | registro bibliográfico |
| رقم الانضمام: | edsbas.AFD53B9D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1469445X 09580670 |
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| DOI: | 10.1113/expphysiol.2008.041152 |