دورية أكاديمية
Genetic variability and integration of Merkel cell polyomavirus in Merkel cell carcinoma.
| العنوان: | Genetic variability and integration of Merkel cell polyomavirus in Merkel cell carcinoma. |
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| المؤلفون: | Martel-Jantin, Claire, Filippone, Claudia, Cassar, Olivier, Peter, M, Tomasic, Gorana, Vielh, Philippe, Brière, Josette, Petrella, Tony, Aubriot-Lorton, Marie-Hélène, Mortier, Laurent, Jouvion, Grégory, Sastre-Garau, Xavier, Robert, Caroline, Gessain, Antoine |
| المساهمون: | Epidémiologie et Physiopathologie des Virus Oncogènes (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur Paris -Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Curie Paris, Département de biologie et pathologie médicales Gustave Roussy, Institut Gustave Roussy (IGR), Laboratoire de recherche translationnelle, Direction de la recherche Gustave Roussy, Institut Gustave Roussy (IGR)-Institut Gustave Roussy (IGR), Service d'anatomo-pathologie Paris, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal Paris, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de pathologie, Service d'anatomie et de cytologie pathologiques, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Service de dermatologie, Centre Hospitalier Régional Universitaire Lille (CHRU Lille), Histopathologie humaine et Modèles animaux, Institut Pasteur Paris, Département de médecine oncologique Gustave Roussy, We thank the Institut Pasteur, the Association pour la Recherche sur le Cancer (A09/1/5041, 2009) and La Ligue Nationale Contre le Cancer (RS10/75-1, 2010, RS11/75-63) for financial support. Claire Martel-Jantin was financially supported by the Ministère de l'Enseignement Supérieur et de la Recherche (MESR) of France. Claudia Filippone was financially supported by the program “DIM Maladies Infectieuses Parasitaires et Nosocomiales Emergentes” from Ile-de-France, and from the European Program “EMPERIE”., European Project: 223498,EC:FP7:HEALTH,FP7-HEALTH-2007-B,EMPERIE(2009) |
| المصدر: | ISSN: 0042-6822. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2012 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | MESH: Aged, 80 and over, MESH: Middle Aged, MESH: Phylogeny, MESH: Polyomavirus Infections/virology, MESH: Skin Neoplasms/virology, MESH: Antigens, Viral, Tumor/genetics, Tumor/metabolism, MESH: Carcinoma, Merkel Cell/virology, MESH: Female, MESH: Genetic Variation, MESH: Humans, MESH: Male, MESH: Merkel cell polyomavirus/genetics, MESH: Merkel cell polyomavirus/isolation & purification, MESH: Merkel cell polyomavirus/physiology, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Merkel cell polyomavirus (MCPyV) is associated to Merkel cell carcinoma (MCC). We studied 113 MCC tumoral skin lesions originating from 97 patients. MCPyV detection was higher in fresh-frozen (FF) biopsies (94%) than in formalin-fixed paraffin-embedded biopsies (39-47%). Mean viral load in FF tumor was of 7.5 copies per cell with a very wide range (0.01-95.4). Nineteen complete sequences of LTAg were obtained, mainly from FF biopsies when the viral load was high. Seventeen showed stop codons, all localized downstream of the pRb protein binding domain. Sequence comparison and phylogenetic analysis showed that all sequences clustered in the large C clade of MCPyV strains. MCPyV integration was demonstrated in 19 out of 27 FF MCC DNA biopsies without evidence of specific host cellular genome integration site. In 13/19 cases, the viral junction was located within the second exon of the LTAg, after the pRB binding domain. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/22342276; info:eu-repo/grantAgreement/EC/FP7/223498/EU/European Management Platform for Emerging and Re-emerging Infectious disease Entities/EMPERIE; pasteur-01370953; https://hal-pasteur.archives-ouvertes.fr/pasteur-01370953Test; PUBMED: 22342276 |
| DOI: | 10.1016/j.virol.2012.01.018 |
| الإتاحة: | https://doi.org/10.1016/j.virol.2012.01.018Test https://hal-pasteur.archives-ouvertes.fr/pasteur-01370953Test |
| حقوق: | http://hal.archives-ouvertes.fr/licences/copyrightTest/ |
| رقم الانضمام: | edsbas.AEA1F5EA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.virol.2012.01.018 |
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