دورية أكاديمية
Lysyl oxidase-like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis
| العنوان: | Lysyl oxidase-like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis |
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| المساهمون: | College of Medicine, Dept. of Pediatrics, SUNG�멐UN CHOI, NARA JEON, HOON YOUNG CHOI, JAE IL SHIN, HYEON JOO JEONG, BEOM JIN LIM, Shin, Jae Il, Lim, Beom Jin, Jeong, Hyeon Joo, Choi, Hoon Young |
| بيانات النشر: | D. A. Spandidos |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Amino Acid Oxidoreductases/analysis, Amino Acid Oxidoreductases/genetics, Animals, Cell Line, Disease Progression, Fibrosis, Gene Expression Regulation, Humans, Kidney/metabolism, Kidney/pathology, Kidney Glomerulus/metabolism, Kidney Glomerulus/pathology, Kidney Tubules/metabolism, Kidney Tubules/pathology, Male, Mice, Podocytes/metabolism, Podocytes/pathology, Renal Insufficiency, Chronic/genetics, Chronic/pathology |
| الوصف: | Tubulointerstitial fibrosis is a common end point of chronic kidney diseases, and preventing its progression is key to avoiding renal failure. Transforming growth factor�뫮� (TGF�뫮�) and associated molecules promote tubulointerstitial fibrosis; however, effective therapies targeting these molecules have yet to be developed. Lysyl oxidase�멿ike 2 (LOXL2), which is involved in invasive growth and metastasis of malignant neoplasms, has recently been reported to serve a key role in hepatic and pulmonary fibrosis. However, little is currently known regarding LOXL2 expression in the kidney and its involvement in tubulointerstitial fibrosis. The present study evaluated LOXL2 expression in human and mouse kidney tissues, as well as in cultured renal cells. LOXL2 protein expression was detected in glomerular capillary loops and tubular epithelial cells in human and mouse kidneys. Glomerular LOXL2 was localized to the cytoplasm of podocytes, as determined by double immunofluorescence microscopy using a podocyte marker (synaptopodin). This result was supported by western blot analysis, which demonstrated that LOXL2 protein expression is present in cultured human podocytes and HK��2 human proximal tubular cells. In addition, the mRNA and protein expression levels of LOXL2 were higher in a mouse model of tubulointerstitial fibrosis compared with in control mice. In addition, immunohistochemistry results demonstrated that LOXL2 is present in the fibrous interstitium and infiltrating mononuclear cells in a mouse model of tubulointerstitial fibrosis. The present study demonstrated that LOXL2 is expressed in compartments of renal tissue, where it appears to contribute to the progression of tubulointerstitial fibrosis. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1791-2997 1791-3004 |
| العلاقة: | MOLECULAR MEDICINE REPORTS; J02261; OAK-2017-03520; https://ir.ymlib.yonsei.ac.kr/handle/22282913/160422Test; T201702351; MOLECULAR MEDICINE REPORTS, Vol.16(3) : 2477-2482, 2017 |
| DOI: | 10.3892/mmr.2017.6918 |
| الإتاحة: | https://doi.org/10.3892/mmr.2017.6918Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/160422Test |
| حقوق: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ |
| رقم الانضمام: | edsbas.ACE473FA |
| قاعدة البيانات: | BASE |
| تدمد: | 17912997 17913004 |
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| DOI: | 10.3892/mmr.2017.6918 |