دورية أكاديمية
A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene.
| العنوان: | A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene. |
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| المؤلفون: | King, M, Pearson, T, Shultz, L D, Leif, J, Bottino, R, Trucco, M, Atkinson, M A, Wasserfall, C, Herold, K C, Woodland, R T, Schmidt, M R, Woda, B A, Thompson, M J, Rossini, A A, Greiner, D L |
| المصدر: | Faculty Research 2000 - 2009 |
| بيانات النشر: | The Mouseion at the JAXlibrary |
| سنة النشر: | 2008 |
| المجموعة: | The Jackson Laboratory: The Mouseion at the JAXlibrary |
| مصطلحات موضوعية: | Diabetes-Mellitus, Disease-Models-Animal, Graft-Rejection, Humans, Interleukin-Receptor-Common-gamma-Subunit, Islets-of-Langerhans, Islets-of-Langerhans-Transplantation, Leukocytes-Mononuclear, Mice, Mice-Inbred-NOD, Mice-SCID, Mice-Transgenic, Mutation, Phenotype |
| الوصف: | Immunodeficient NOD-scid mice bearing a targeted mutation in the IL2 receptor common gamma chain (Il2rgamma(null)) readily engraft with human stem cells. Here we analyzed human peripheral blood mononuclear cells (PBMC) for their ability to engraft NOD-scid Il2rgamma(null) mice and established engraftment kinetics, optimal cell dose, and the influence of injection route. Even at low PBMC input, NOD-scid Il2rgamma(null) mice reproducibly support high human PBMC engraftment that plateaus within 3-4 weeks. In contrast to previous stocks of immunodeficient mice, we observed low intra- and inter-donor variability of engraftment. NOD-scid Il2rgamma(null) mice rendered hyperglycemic by streptozotocin treatment return to normoglycemia following transplantation with human islets. Interestingly, these human islet grafts are rejected following injection of HLA-mismatched human PBMC as evidenced by return to hyperglycemia and loss of human C-peptide. These data suggest that humanized NOD-scid Il2rgamma(null) mice may represent an important surrogate for investigating in vivo mechanisms of human islet allograft rejection. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://mouseion.jax.org/stfb2000_2009/1730Test; http://dx.doi.org/doi:10.1016/j.clim.2007.11.001Test |
| DOI: | 10.1016/j.clim.2007.11.001 |
| الإتاحة: | https://doi.org/10.1016/j.clim.2007.11.001Test https://mouseion.jax.org/stfb2000_2009/1730Test |
| رقم الانضمام: | edsbas.ACD94D8D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.clim.2007.11.001 |
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