التفاصيل البيبلوغرافية
| العنوان: |
A Cost‐Effective Hemin‐Based Artificial Enzyme Allows for Practical Applications |
| المؤلفون: |
Qiu, Dehui, He, Fangni, Liu, Yuan, Zhou, Zhaoxi, Yang, Yuqin, Long, Zhongwen, Chen, Qianqian, Chen, Desheng, Wei, Shijiong, Mao, Xuanxiang, Zhang, Xiaobo, Mergny, Jean‐Louis, Monchaud, David, Ju, Huangxian, Zhou, Jun |
| المساهمون: |
National Natural Science Foundation of China, State Key Laboratory of Analytical Chemistry for Life Science, Fundamental Research Funds for the Central Universities |
| المصدر: |
Advanced Science ; ISSN 2198-3844 2198-3844 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2024 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Nanomaterials excel in mimicking the structure and function of natural enzymes while being far more interesting in terms of structural stability, functional versatility, recyclability, and large‐scale preparation. Herein, the story assembles hemin, histidine analogs, and G‐quadruplex DNA in a catalytically competent supramolecular assembly referred to as assembly‐activated hemin enzyme (AA‐heminzyme). The catalytic properties of AA‐heminzyme are investigated both in silico (by molecular docking and quantum chemical calculations) and in vitro (notably through a systematic comparison with its natural counterpart horseradish peroxidase, HRP). It is found that this artificial system is not only as efficient as HRP to oxidize various substrates (with a turnover number k cat of 115 s −1 ) but also more practically convenient (displaying better thermal stability, recoverability, and editability) and more economically viable, with a catalytic cost amounting to <10% of that of HRP. The strategic interest of AA‐heminzyme is further demonstrated for both industrial wastewater remediation and biomarker detection (notably glutathione, for which the cost is decreased by 98% as compared to commercial kits). |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/advs.202402237 |
| الإتاحة: |
https://doi.org/10.1002/advs.202402237Test |
| حقوق: |
http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.ACBC6490 |
| قاعدة البيانات: |
BASE |
