دورية أكاديمية
Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes
| العنوان: | Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes |
|---|---|
| المؤلفون: | Patricia H. Alvarenga, Thiago Luiz Alves e Silva, Motoshi Suzuki, Glenn Nardone, Pedro Cecilio, Joel Vega-Rodriguez, Jose M.C. Ribeiro, John F. Andersen |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Physiology, Ecology, Immunology, Inorganic Chemistry, Biological Sciences not elsewhere classified, free quantification approaches, families classically described, applied mass spectrometry, aedes aegypti <, 2 %, respectively, comprehensive proteomics analysis, also highly abundant, 102 serine proteases, based absolute quantification, serine protease, comprehensive catalogue, also provides, transports nutrients, relative abundances, proteins belonging, physiological importance, mosquito physiology, male mosquitoes, immune response, future studies, future research, disease transmission |
| الوصف: | In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1403 proteins were identified, out of which 447 of them were predicted to be extracellular. In both sexes, almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundances (based on their intensity-based absolute quantification, iBAQ) were 37.9 and 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues were identified, with almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, such as odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of A. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on hemolymph composition. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/journal_contribution/Comprehensive_Proteomics_Analysis_of_the_Hemolymph_Composition_of_Sugar-Fed_i_Aedes_aegypti_i_Female_and_Male_Mosquitoes/25342872Test |
| DOI: | 10.1021/acs.jproteome.3c00918.s001 |
| الإتاحة: | https://doi.org/10.1021/acs.jproteome.3c00918.s001Test https://figshare.com/articles/journal_contribution/Comprehensive_Proteomics_Analysis_of_the_Hemolymph_Composition_of_Sugar-Fed_i_Aedes_aegypti_i_Female_and_Male_Mosquitoes/25342872Test |
| حقوق: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.ACBC34F6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jproteome.3c00918.s001 |
|---|