دورية أكاديمية
Changes in searching behaviour of CSL transcription complexes in Notch active conditions.
| العنوان: | Changes in searching behaviour of CSL transcription complexes in Notch active conditions. |
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| المؤلفون: | Baloul, Sarah, Roussos, Charalambos, Gomez-Lamarca, Maria, Muresan, Leila, Bray, Sarah |
| بيانات النشر: | Life Science Alliance, LLC Department of Physiology, Development and Neuroscience //dx.doi.org/10.26508/lsa.202302336 Life Sci Alliance |
| سنة النشر: | 2024 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Animals, DNA-Binding Proteins, Receptors, Notch, Transcription Factors, Gene Expression Regulation, Appetitive Behavior |
| الوصف: | During development cells receive a variety of signals, which are of crucial importance to their fate determination. One such source of signal is the Notch signalling pathway, where Notch activity regulates expression of target genes through the core transcription factor CSL. To understand changes in transcription factor behaviour that lead to transcriptional changes in Notch active cells, we have probed CSL behaviours in real time, using in vivo Single Molecule Localisation Microscopy. Trajectory analysis reveals that Notch-On conditions increase the fraction of bound CSL molecules, but also the proportion of molecules with exploratory behaviours. These properties are shared by the co-activator Mastermind. Furthermore, both CSL and Mastermind, exhibit characteristics of local exploration near a Notch target locus. A similar behaviour is observed for CSL molecules diffusing in the vicinity of other bound CSL clusters. We suggest therefore that CSL acquires an exploratory behaviour when part of the activation complex, favouring local searching and retention close to its target enhancers. This change explains how CSL can efficiently increase its occupancy at target sites in Notch-On conditions. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/362005.2Test |
| الإتاحة: | https://www.repository.cam.ac.uk/handle/1810/362005.2Test |
| حقوق: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.AC581C76 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |