| الوصف: |
Recent studies in PKU patients identified alternative biomarkers in blood using untargeted metabolomics. To test the added clinical value of these novel biomarkers, targeted metabolomics of 11 PKU biomarkers (phenylalanine, glutamyl‐phenylalanine, glutamyl‐glutamyl‐phenylalanine, N‐lactoyl‐phenylalanine, N‐acetyl‐phenylalanine, the dipeptides phenylalanyl‐phenylalanine and phenylalanyl‐leucine, phenylalanine–hexose conjugate, phenyllactate, phenylpyruvate, and phenylacetate) was performed in stored serum samples of the well‐defined PKU patient‐COBESO cohort and a healthy control group. Serum samples of 35 PKU adults and 20 healthy age‐ and sex‐matched controls were analyzed using ultra‐high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry. Group differences were tested using the Mann–Whitney U test. Multiple linear regression analyses were performed with these biomarkers as predictors of (neuro‐)cognitive functions working memory, sustained attention, inhibitory control, and mental health. Compared to healthy controls, phenylalanine, glutamyl‐phenylalanine, N‐lactoyl‐phenylalanine, N‐acetyl‐phenylalanine, phenylalanine–hexose conjugate, phenyllactate, phenylpyruvate, and phenylacetate were significant elevated in PKU adults ( p < 0.001). The remaining three were below limit of detection in PKU and controls. Both phenylalanine and N‐lactoyl‐phenylalanine were associated with DSM‐VI Attention deficit/hyperactivity ( R 2 = 0.195, p = 0.039 and R 2 = 0.335, p = 0.002, respectively) of the ASR questionnaire. In addition, N‐lactoyl‐phenylalanine showed significant associations with ASR DSM‐VI avoidant personality ( R 2 = 0.265, p = 0.010), internalizing ( R 2 = 0.192, p = 0.046) and externalizing problems ( R 2 = 0.217, p = 0.029) of the ASR questionnaire and multiple aspects of the MS2D and FI tests, reflecting working memory with R 2 between 0.178 ( p = 0.048) and 0.204 ( p = 0.033). Even though the strength of the models was not considered strong, N‐lactoyl‐phenylalanine ... |
