دورية أكاديمية
Therapeutic Effect of Resveratrol on Oxidative Stress in Graves' Orbitopathy Orbital Fibroblasts
| العنوان: | Therapeutic Effect of Resveratrol on Oxidative Stress in Graves' Orbitopathy Orbital Fibroblasts |
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| المساهمون: | Chang Yeom Kim, Hyun Jung Lee, Min Kyung Chae, Jung Woo Byun, Eun Jig Lee, Jin Sook Yoon, Kim, Chang Yeom, Byun, Jung Woo, Yoon, Jin Sook, Lee, Eun Jig |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adipocytes/metabolism, Adipocytes/pathology, Adipogenesis/drug effects, Angiogenesis Inhibitors, Antioxidants/therapeutic use, Cell Proliferation, Cells, Cultured, Fibroblasts/metabolism, Fibroblasts/pathology, Graves Ophthalmopathy/drug therapy, Graves Ophthalmopathy/metabolism, Graves Ophthalmopathy/pathology, Humans, Oxidative Stress/drug effects, Reactive Oxygen Species/metabolism, Stilbenes/therapeutic use, adipogenesis, Graves�� orbitopathy, orbital fibroblast, oxidative stress, reactive oxygen species, resveratrol |
| الوصف: | PURPOSE: Multiple causative factors complicate the pathogenesis in Graves' orbitopathy (GO). It has been suggested that oxidative stress contributes to the development and progression of GO. Therefore, we investigated the therapeutic effect of resveratrol, a potent antioxidant, upon oxidative stress levels in GO orbital fibroblasts in vitro. METHODS: Orbital fibroblasts were cultured from orbital connective tissues obtained from GO patients. Intracellular reactive oxygen species (ROS) levels and the expression of heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and thioredoxin (Trx), were measured after resveratrol treatment. Adipogenesis was induced, and ROS levels were examined during adipogenic differentiation. Western blot assay was performed to evaluate the effects of resveratrol on the expression of antioxidants levels and transcriptional regulators. RESULTS: Treatment with 30 or 50 關M resveratrol reduced ROS production and HO-1 level induced by oxidative stress. Levels of Cu/Zn-SOD, catalase, and Trx were also reduced, while Mn-SOD increased with 50 關M resveratrol treatment. Resveratrol suppressed adipogenesis, reducing the number of adipocytes and suppressing the accumulation of lipid droplets. Treatment with 50 關M resveratrol also decreased ROS levels during adipogenesis. Expression of the transcriptional regulators phosphor-extracellular signal-regulated kinase and phospho-c-Jun NH(2)-terminal kinase significantly increased after treatment with 50 關M resveratrol, and decreased in response to inhibitors of each protein. Phosphonuclear factor kappa-light-chain-enhancer of activated B cells p65 levels also increased after treatment with 50 關M resveratrol. CONCLUSIONS: Resveratrol reduced ROS levels and inhibited adipogenesis in GO orbital fibroblasts in vitro. This study supports the potential use of resveratrol in GO treatment. ; open |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 6352~6361 |
| اللغة: | unknown |
| تدمد: | 0146-0404 1552-5783 |
| العلاقة: | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE; J01187; OAK-2015-02250; https://ir.ymlib.yonsei.ac.kr/handle/22282913/141410Test; http://iovs.arvojournals.org/article.aspx?articleid=2454873Test; T201503880; INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.56(11) : 6352-6361, 2015 |
| DOI: | 10.1167/iovs.15-16870 |
| الإتاحة: | https://doi.org/10.1167/iovs.15-16870Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/141410Test http://iovs.arvojournals.org/article.aspx?articleid=2454873Test |
| حقوق: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ ; not free |
| رقم الانضمام: | edsbas.ABBB7510 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 01460404 15525783 |
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| DOI: | 10.1167/iovs.15-16870 |