دورية أكاديمية
Determination of the genetic diversity of different bioluminescent bacteria by pulsed-field gel electrophoresis (PFGE)
| العنوان: | Determination of the genetic diversity of different bioluminescent bacteria by pulsed-field gel electrophoresis (PFGE) |
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| المؤلفون: | Omeroglu E.E. |
| المساهمون: | Ege Üniversitesi |
| بيانات النشر: | Kowsar Medical Publishing Company |
| سنة النشر: | 2015 |
| المجموعة: | Ege University Institutional Repository |
| مصطلحات موضوعية: | Aliivibrio, Bioluminescent, Photobacterium, Pulsed-Field Gel Electrophoresis, Shewanella, Vibrio |
| الوصف: | Background: There are 4 different genera (i.e. Vibrio, Aliivibrio, Photobacterium, and Shewanella) in the new classification of bioluminescent bacteria. The mechanism of bioluminescence has yet to be fully elucidated. Therefore, the determination of physiological and genetic characteristics of bioluminescent bacteria isolated from different sources is very important. Pulsed-Field Gel Electrophoresis (PFGE) has the highest discriminatory power among the different molecular typing methods for the investigation of the clonal relationships between bacteria. For the PFGE analysis of bioluminescent bacteria, the NotI-HF™ is the method of choice among the restriction enzymes. Objectives: The present study aimed to determine genetic relatedness via PFGE in 41 bioluminescent bacteria (belonging to 10 different species) isolated and identified from various marine sources. Materials and Methods: Diferent bioluminescent bacteria (i.e. Vibrio gigantis, V. azureus, V. harveyi, V. lentus, V. crassostreae, V. orientalis, Aliivibrio logei, A. fscheri, Shewanella woodyi, and Photobacterium kishitanii) were analyzed by PFGE using the NotI-HF™ restriction enzyme. The whole DNA of the strains embedded into the agarose plugs was digested with enzyme at 37°C for 30 minutes. CHEF-Mapper PFGE system was used for electrophoresis and band profle of the strains for the NotI-HF™ restriction enzyme were analyzed by Bio-Profl-1D++ software (Vilber Lourmat) at 10% homology coefficient. Results: Although all experiments were performed three times, four of forty-one bioluminescent strains (V. gigantis E-16, H-16 and S3W46 strains and A. fscheri E-4 strain) could not be typed by PFGE technique with NotI-HF™ enzyme. While only two strains (V. crassostreae H-12 and H-19 strains) were exhibiting same band pattern profiles (100% genome homology), thirty-six different PFGE band patterns were obtained. Pattern homologies changed between 66% - 92%, 73% - 83% and 49% - 100% for V. gigantis, V. harveyi and other strains, respectively. Conclusions: The ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2008-3645 |
| العلاقة: | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; Jundishapur Journal of Microbiology; https://doi.org/10.5812/jjm.28378v2Test; https://hdl.handle.net/11454/17139Test |
| DOI: | 10.5812/jjm.28378v2 |
| الإتاحة: | https://doi.org/10.5812/jjm.28378v2Test https://hdl.handle.net/11454/17139Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.AB67B5B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20083645 |
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| DOI: | 10.5812/jjm.28378v2 |