التفاصيل البيبلوغرافية
| العنوان: |
The PI3K/AKT/mTOR signaling pathway is aberrantly activated in primary central nervous system lymphoma and correlated with a poor prognosis |
| المؤلفون: |
Zhang, Xiaowei, Wu, Yuchen, Sun, Xuefei, Cui, Qu, Bai, Xueyan, Dong, Gehong, Gao, Zifen, Wang, Yaming, Gao, Chunji, Sun, Shengjun, Ji, Nan, Liu, Yuanbo |
| المصدر: |
BMC Cancer ; volume 22, issue 1 ; ISSN 1471-2407 |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Cancer Research, Genetics, Oncology |
| الوصف: |
Background Primary central nervous system lymphoma (PCNSL) is a specific subtype of non-Hodgkin lymphoma that is highly invasive and confined to the central nervous system (CNS). The vast majority of PCNSLs are diffuse large B-cell lymphomas (DLBCLs). PCNSL is a highly heterogeneous disease, and its pathogenesis has not yet been fully elucidated. Further studies are needed to guide individualized therapy and improve the prognosis. Methods In this study, we detected 1) the expression of p-AKT, p-mTOR, p-S6 and p-4E-BP1 by immunohistochemistry (IHC) and Western blotting, 2) the mRNA expression by real-time qPCR and 3) the deletion of PTEN gene by immunofluorescence in situ hybridization (FISH) in order to investigate the activation status of the PI3K/AKT/mTOR signaling pathway in PCNSL. Samples of reactive hyperplasia lymphnods were used as the control group. The correlations between the clinical characteristics and prognosis of PCNSL patients and the expression of p-AKT, p-mTOR, p-S6 and p-4E-BP1 and the deletion of PTEN were assessed. Results The IHC results showed that the positive expression rates of p-AKT, p-mTOR, p-S6 and p-4E-BP1 in PCNSL were significantly higher in the PCNSL group than in the control group ( P < 0.05). The relative mRNA expression level of MTOR in PCNSL samples was significantly increased ( P = 0.013). Correlation analysis revealed that the expression of p-mTOR was correlated with that of p-AKT, p-S6, p-4E-BP1. PTEN deletion was found in 18.9% of PCNSL samples and was correlated with the expression of p-AKT ( P = 0.031). Correlation analysis revealed that the PCNSL relapse rate in the p-mTOR-positive group was 64.5%, significantly higher than that in the negative group ( P = 0.001). Kaplan-Meier survival analysis showed inferior progression-free survival (PFS) in the p-mTOR- and p-S6-positive groups ( P = 0.002 and 0.009, respectively), and PTEN deletion tended to be related to shorter overall survival (OS) ( P = 0.072). Cox regression analysis revealed p-mTOR expression as an ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1186/s12885-022-09275-z |
| DOI: |
10.1186/s12885-022-09275-z.pdf |
| DOI: |
10.1186/s12885-022-09275-z/fulltext.html |
| الإتاحة: |
https://doi.org/10.1186/s12885-022-09275-zTest |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: |
edsbas.AA459385 |
| قاعدة البيانات: |
BASE |
