صورة
FIGURE 2 from FBXW7 -loss Sensitizes Cells to ATR Inhibition Through Induced Mitotic Catastrophe
| العنوان: | FIGURE 2 from FBXW7 -loss Sensitizes Cells to ATR Inhibition Through Induced Mitotic Catastrophe |
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| المؤلفون: | Siobhan O'Brien, Tajinder Ubhi, Lucie Wolf, Krishna Gandhi, Sichun Lin, Naz Chaudary, Neesha C. Dhani, Michael Milosevic, Grant W. Brown, Stephane Angers |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cancer, Cancer Biology, Molecular and Cellular Biology, Therapeutic Research and Development, Clinical Research and Trials, Oncogenes & Tumor Suppressors, Tumor suppressor genes, Cell Cycle, Control of cell cycle progression, Cell cycle checkpoints, Dna Damage And Repair, DNA repair, Drug Mechanisms, Cellular responses to anticancer drugs, Small Molecule Agents, Kinase inhibitors, Precision Medicine |
| الوصف: | FBXW7 − / − cells have high levels of replication stress and a reduced replication fork rate. A, Immunofluorescence of markers of DNA damage (γH2AX, 53BP1) and replication stress (RPA32) are increased in the FBXW7 − / − cells compared with wild-type. Representative images of three replicate experiments, scale bar 20 µm. B, Quantification of immunofluorescence images, combination of three replicate experiments, mean ± SEM, unpaired t test. C, Schematic of pulsing strategy used for DNA combing of wild-type and FBXW7 − / − HPAF-II cells. D, Representative images DNA combing experiment highlighting CldU and IdU incorporation. E, Quantification of DNA combing experiment in D measuring replication fork rate, unpaired t test mean ± SEM P < 0.001, minimum of 224 replication tracks were quantified per sample. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| العلاقة: | https://figshare.com/articles/figure/FIGURE_2_from_i_FBXW7_i_-loss_Sensitizes_Cells_to_ATR_Inhibition_Through_Induced_Mitotic_Catastrophe/24886234Test |
| DOI: | 10.1158/2767-9764.24886234.v1 |
| الإتاحة: | https://doi.org/10.1158/2767-9764.24886234.v1Test https://figshare.com/articles/figure/FIGURE_2_from_i_FBXW7_i_-loss_Sensitizes_Cells_to_ATR_Inhibition_Through_Induced_Mitotic_Catastrophe/24886234Test |
| حقوق: | CC BY |
| رقم الانضمام: | edsbas.AA065030 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1158/2767-9764.24886234.v1 |
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