مؤتمر
Glutamicibacter creatinolyticus strain LGCM 259 infection in a horse: first genetic characterization
| العنوان: | Glutamicibacter creatinolyticus strain LGCM 259 infection in a horse: first genetic characterization |
|---|---|
| المؤلفون: | Attili, Annarita, Gonçalves dos Santos, Roselane, Hurtado Castillo, Raquel, Gabriel, Lucas, Profeta, Rodrigo, Rifici, Claudia, Mazzullo, Giuseppe, Spier Sharon, J., Pinto Gomide Anne, Cybelle, Brenig, Bertram, Gala-García, Alfonso, de Paula Castro Thiago, Luiz, Cuteri, Vincenzo, Azevedo, Vasco, Seyffert, Núbia |
| المساهمون: | Attili, Annarita, Gonçalves dos Santos, Roselane, Hurtado Castillo, Raquel, Gabriel, Luca, Profeta, Rodrigo, Rifici, Claudia, Mazzullo, Giuseppe, Spier Sharon, J., Pinto Gomide Anne, Cybelle, Brenig, Bertram, Gala-García, Alfonso, de Paula Castro Thiago, Luiz, Cuteri, Vincenzo, Azevedo, Vasco, Seyffert, Núbia |
| بيانات النشر: | European College of Veterinary Microbiology (ECVM) GRC Athens (Greece) |
| سنة النشر: | 2019 |
| المجموعة: | CAMPUS Pubblicazioni Scientifiche Unicam (Università di Camerino) |
| الوصف: | Glutamicibacter creatinolyticus (formerly Arthrobacter creatinolyticus) belonged to the family Microccaceae. It plays a significant role in many ecosystems: soil, water, air, cheese, plant, and it was associated to urinary tract infections and bacteremia in humans [1]. Recently, G. creatinolyticus LGCM 259 (LGCM-259) was isolated from diffuse subcutaneous nodules adherent to muscular tissues from a mare in Italy. Objectives Since the identification is only possible using 16S rRNA and MALDI- TOF sequence analyses11, this study was carried out to: -characterize LGCM-259 with a complete DNA sequence and annotation; -identify genes encoding virulence factors. Materials and methods By comparative analyses among four isolated species of different habitats, available in the NCBI database, chromosomal sequencing using Hiseq technology (Illumina, USA) was conducted. The genome of LGCM-259 was automatically annotated using PROKKA. A phylogenomic tree and the presence of virulence genes were generated by Phylogenomic Tree Tool in Pathosystems Resource Integration Center (version-3.5.17), and BLASTp against the Virulence Factor Database (VFDB), respectively. Results LGCM-259 strain was sequenced and assembled in a circular chromosome, which exhibits a length of 3.3 Mb, with a G+C content of 66.4%, and a total of 2882 CDSs, 4 clusters of rRNAS (5S, 16S, and 23S), and 61 tRNA genes, respectively. The locus tag LGCM259_1698, LGCM259_0905 and LGCM259_1698 may be involved in multiple drug resistance to Rifampin, Elfamycin, and Fluoroquinolone (rpoB, tufA, tufB). The genome also displayed copper tolerance genes (copZ, csoR_1, cutC, aniA, pcoC), resistance to heavy metals such as arsenic, cobalt-zinc-cadmium (cobT, cobS, cadA), and chrome composts which are serious environmental contaminants. Conclusion The LGCM 259 strain’s genome was first characterize and its chromosome sequence has been deposited in the NCBI database under accession number CP034412. It affects animals and carries important bacterial virulence factors ... |
| نوع الوثيقة: | conference object |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/isbn/9876543210; ispartofbook:Abstract Book of The 1st International Conference of the European College of Veterinary Microbiology; The 1st International Conference of the European College of Veterinary Microbiology; volume:1; firstpage:68; lastpage:68; numberofpages:1; http://hdl.handle.net/11581/434779Test; https://ebvs.eu/news/1st-international-conference-of-the-european-college-of-veterinary-microbiologyTest |
| الإتاحة: | http://hdl.handle.net/11581/434779Test https://ebvs.eu/news/1st-international-conference-of-the-european-college-of-veterinary-microbiologyTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A9E1262C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |