التفاصيل البيبلوغرافية
| العنوان: |
Effects of PDE4 pathway inhibition in rat experimental stroke. |
| المؤلفون: |
Yang, Fan, Sumbria, Rachita K, Xue, Dong, Yu, Chuanhui, He, Dan, Liu, Shuo, Paganini-Hill, Annlia, Fisher, Mark |
| المصدر: |
Journal of Pharmacy & Pharmaceutical Sciences, vol 17, iss 3 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2014 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Pharmacology and Pharmaceutical Sciences, Biomedical and Clinical Sciences, Brain Disorders, Stroke, Aetiology, 2.1 Biological and endogenous factors, Animals, Cyclic Nucleotide Phosphodiesterases, Type 4, Disease Models, Animal, Fibrinolysis, Fluorescent Antibody Technique, Male, Microvessels, Oligonucleotide Array Sequence Analysis, Phosphodiesterase 4 Inhibitors, Rats, Inbred F344, Wistar, phosphodiesterase IV, rolipram, phosphodiesterase IV inhibitor, animal experiment, animal model, animal tissue, article, brain infarction size, brain ischemia, controlled study |
| جغرافية الموضوع: |
362 - 370 |
| الوصف: |
PurposeThe first genomewide association study indicated that variations in the phosphodiesterase 4D (PDE4D) gene confer risk for ischemic stroke. However, inconsistencies among the studies designed to replicate the findings indicated the need for further investigation to elucidate the role of the PDE4 pathway in stroke pathogenesis. Hence, we studied the effect of global inhibition of the PDE4 pathway in two rat experimental stroke models, using the PDE4 inhibitor rolipram. Further, the specific role of the PDE4D isoform in ischemic stroke pathogenesis was studied using PDE4D knockout rats in experimental stroke.MethodsRats were subjected to either the ligation or embolic stroke model and treated with rolipram (3mg/kg; i.p.) prior to the ischemic insult. Similarly, the PDE4D knockout rats were subjected to experimental stroke using the embolic model.ResultsGlobal inhibition of the PDE4 pathway using rolipram produced infarcts that were 225% (p<0.01) and 138% (p<0.05) of control in the ligation and embolic models, respectively. PDE4D knockout rats subjected to embolic stroke showed no change in infarct size compared to wild-type control.ConclusionsDespite increase in infarct size after global inhibition of the PDE4 pathway with rolipram, specific inhibition of the PDE4D isoform had no effect on experimental stroke. These findings support a role for the PDE4 pathway, independent of the PDE4D isoform, in ischemic stroke pathogenesis. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt7084z167; https://escholarship.org/uc/item/7084z167Test |
| الإتاحة: |
https://escholarship.org/uc/item/7084z167Test |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.A99FF976 |
| قاعدة البيانات: |
BASE |
