دورية أكاديمية
The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
| العنوان: | The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells |
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| المؤلفون: | Pospíšilová, Veronika, Ešner, Milan, Červenková, Iveta, Fedr, Radek, Tinevez, Jean-Yves, Hampl, Aleš, Anger, Martin |
| المساهمون: | Department of Histology and Embryology Brno (MED / MUNI), Faculty of Medicine Brno (MED / MUNI), Masaryk University Brno (MUNI)-Masaryk University Brno (MUNI), Central European Institute of Technology Brno (CEITEC MU), Brno University of Technology Brno (BUT), Institute of Biophysics of the Czech Academy of Sciences (IBP / CAS), Czech Academy of Sciences Prague (CAS), Hub d'analyse d'images - Image Analysis Hub (Platform) (IAH), Institut Pasteur Paris (IP), St. Anne’s University Hospital Brno, This work was supported by Czech Science Foundation projects 15-11707S and 15-04844S andthe European Regional Development Fund-Project “National infrastructure for biological and medical imaging” (No.CZ.02.1.01/0.0/0.0/16_013/000 1775)., We thank Drs. Anthony Hyman and Ina Poser (MPICBG, Dresden, Germany) for providing the H2A-GFP BAC construct, Dr. Ronald Naumann (MPI-CBG, Dresden, Germany) for providing the JM8.A mouse ES cells, and Dr. Klara Koudelkova for her excellent technical assistance |
| المصدر: | ISSN: 1214-021X. |
| بيانات النشر: | HAL CCSD University of South Bohemia in České Budějovice |
| سنة النشر: | 2019 |
| المجموعة: | Institut Pasteur: HAL |
| مصطلحات موضوعية: | Mitosis length, Multipolar division, Embryonic stem (ES) cells, Single-cell tracking, Spindle assembly checkpoint (SAC), Time-lapse microscopy, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their clinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | pasteur-02616481; https://hal-pasteur.archives-ouvertes.fr/pasteur-02616481Test; https://hal-pasteur.archives-ouvertes.fr/pasteur-02616481/documentTest; https://hal-pasteur.archives-ouvertes.fr/pasteur-02616481/file/The%20frequency%20and%20consequences%20of%20multipolar%20mitoses%20in%20undifferentiated%20embryonic%20stem%20cells.pdfTest |
| DOI: | 10.32725/jab.2019.018 |
| الإتاحة: | https://doi.org/10.32725/jab.2019.018Test https://hal-pasteur.archives-ouvertes.fr/pasteur-02616481Test https://hal-pasteur.archives-ouvertes.fr/pasteur-02616481/documentTest https://hal-pasteur.archives-ouvertes.fr/pasteur-02616481/file/The%20frequency%20and%20consequences%20of%20multipolar%20mitoses%20in%20undifferentiated%20embryonic%20stem%20cells.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-nc-ndTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.A96C0458 |
| قاعدة البيانات: | BASE |
| DOI: | 10.32725/jab.2019.018 |
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