دورية أكاديمية
Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesis
| العنوان: | Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesis |
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| المؤلفون: | Errede M., Mangieri D., Longo G., Girolamo F., De Trizio I., Vimercati A., Serio G., Frei K., Perris R., Virgintino D. |
| المساهمون: | Errede, M., Mangieri, D., Longo, G., Girolamo, F., De Trizio, I., Vimercati, A., Serio, G., Frei, K., Perris, R., Virgintino, D. |
| سنة النشر: | 2018 |
| المجموعة: | Università degli Studi di Foggia: CINECA IRIS Institutional Research Information System |
| مصطلحات موضوعية: | Angiogenesi, Cell-to-cell communication, Developing cerebral cortex, Glioblastoma, Pericyte, Tunneling nanotube, Adult, Brain Neoplasm, Cell Communication, Cells, Cultured, Cerebral Cortex, Endothelial Cell, Female, Human, Male, Middle Aged, Nanotube, Neovascularization, Pathologic, Physiologic |
| الوصف: | Background: Nanotubular structures, denoted tunneling nanotubes (TNTs) have been described in recent times as involved in cell-to-cell communication between distant cells. Nevertheless, TNT-like, long filopodial processes had already been described in the last century as connecting facing, growing microvessels during the process of cerebral cortex vascularization and collateralization. Here we have investigated the possible presence and the cellular origin of TNTs during normal brain vascularization and also in highly vascularized brain tumors. Methods: We searched for TNTs by high-resolution immunofluorescence confocal microscopy, applied to the analysis of 20-μm, thick sections from lightly fixed, unembedded samples of both developing cerebral cortex and human glioblastoma (GB), immunolabeled for endothelial, pericyte, and astrocyte markers, and vessel basal lamina molecules. Results: The results revealed the existence of pericyte-derived TNTs, labeled by proteoglycan NG2/CSPG4 and CD146. In agreement with the described heterogeneity of these nanostructures, ultra-long (> 300 μm) and very thin (< 0.8 μm) TNTs were observed to bridge the gap between the wall of distant vessels, or were detected as short (< 300 μm) bridging cables connecting a vessel sprout with its facing vessel or two apposed vessel sprouts. The pericyte origin of TNTs ex vivo in fetal cortex and GB was confirmed by in vitro analysis of brain pericytes, which were able to form and remained connected by typical TNT structures. Conclusions: None of the multiple roles described for TNTs can be excluded from a possible involvement during the processes of both normal and pathological vessel growth. A possible function, suggested by the pioneering studies made during cerebral cortex vascularization, is in cell searching and cell-to-cell recognition during the processes of vessel collateralization and vascular network formation. According to our results, it is definitely the pericyte-derived TNTs that seem to actively explore the surrounding ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/30290761; info:eu-repo/semantics/altIdentifier/wos/WOS:000446461900001; volume:15; issue:1; firstpage:28; journal:FLUIDS AND BARRIERS OF THE CNS; http://hdl.handle.net/11369/389046Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85054461096; http://www.cerebrospinalfluidresearch.comTest/ |
| DOI: | 10.1186/s12987-018-0114-5 |
| الإتاحة: | https://doi.org/10.1186/s12987-018-0114-5Test http://hdl.handle.net/11369/389046Test http://www.cerebrospinalfluidresearch.comTest/ |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A80A4C85 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12987-018-0114-5 |
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