دورية أكاديمية
Total serum pentosidine quantification using liquid chromatography-tandem mass spectrometry
| العنوان: | Total serum pentosidine quantification using liquid chromatography-tandem mass spectrometry |
|---|---|
| المؤلفون: | Lindsie A. Blencowe, Andrea Božović, Evelyn Wong, Vathany Kulasingam, Angela M. Cheung |
| المصدر: | Bone Reports, Vol 20, Iss , Pp 101737- (2024) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Pentosidine, Liquid chromatography-tandem mass spectrometry, Advanced glycation end product, Osteoporosis, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Pentosidine (PEN) is an Advanced Glycation End-product (AGE) that is known to accumulate in bone collagen with aging and contribute to fracture risk. The PEN content in bone is correlated with serum PEN, making it an attractive, potential osteoporosis biomarker. We sought to develop a method for quantifying PEN in stored serum. After conducting a systematic narrative review of PEN quantification methodologies, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantifying total serum PEN. Our method is both sensitive and precise (LOD 2 nM, LOQ 5 nM, %CV < 6.5 % and recovery 91.2–100.7 %). Our method is also equivalent or better than other methods identified in our review. Additionally, LC-MS/MS avoids the pitfalls and limitations of using fluorescence as a means of detection and could be adapted to investigate a broad range of AGE compounds. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2352-1872 |
| العلاقة: | http://www.sciencedirect.com/science/article/pii/S2352187224000044Test; https://doaj.org/toc/2352-1872Test; https://doaj.org/article/8680c46fed064839891147bc5b1c35baTest |
| DOI: | 10.1016/j.bonr.2024.101737 |
| الإتاحة: | https://doi.org/10.1016/j.bonr.2024.101737Test https://doaj.org/article/8680c46fed064839891147bc5b1c35baTest |
| رقم الانضمام: | edsbas.A793E119 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23521872 |
|---|---|
| DOI: | 10.1016/j.bonr.2024.101737 |