التفاصيل البيبلوغرافية
| العنوان: |
Mapping of Phospholipids by MALDI Imaging (MALDI-MSI): Realities and Expectations. ; Chem Phys Lipids |
| المساهمون: |
Sparvero, L.J., Amoscato, A.A., Dixon, C.E., Long, J.B., Kochanek, P.M., Pitt, B.R., Bayir, H., Kagan, V.E. |
| المصدر: |
Chem Phys Lipids. 2012; 165(5):545-562. |
| المجموعة: |
CDC Stacks (Centers for Disease Control and Prevention) |
| مصطلحات موضوعية: |
Article, Lipidomics, MALDI imaging, Traumatic brain injury, Acute lung injury, Lipid peroxidation, Docosahexaenoic acid, Brain, Dietary Supplements, Docosahexaenoic Acids, Molecular Imaging, Phospholipids, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| الوصف: |
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges. ; ES020693/ES/NIEHS NIH HHS/United States ; ES021068/ES/NIEHS NIH HHS/United States ; HL094488/HL/NHLBI NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; NS076511/NS/NINDS NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; P30 CA047904/CA/NCI NIH HHS/United States ; P30 CA047904/CA/NCI NIH HHS/United States ; P50 NS030318/NS/NINDS NIH HHS/United States ; R01 ES020693/ES/NIEHS NIH HHS/United States ; R01 HL070755/HL/NHLBI NIH HHS/United States ; R01 HL094488/HL/NHLBI NIH HHS/United States ; R01 NS061817/NS/NINDS NIH HHS/United States ; R01 NS076511/NS/NINDS NIH HHS/United States ; R21 ES021068/ES/NIEHS NIH HHS/United States ; R37 HL065697/HL/NHLBI NIH HHS/United States ; U19 AI068021/AI/NIAID NIH HHS/United States ; U19AI068021/AI/NIAID NIH HHS/United States ; 2013-07-01T00:00:00Z ; 22692104 ; PMC3642772 |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
unknown |
| العلاقة: |
cdc:33549; http://stacks.cdc.gov/view/cdc/33549Test/ |
| الإتاحة: |
http://stacks.cdc.gov/view/cdc/33549Test/ |
| رقم الانضمام: |
edsbas.A7068003 |
| قاعدة البيانات: |
BASE |
