دورية أكاديمية
Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase.
| العنوان: | Checkpoint inhibition of origin firing prevents inappropriate replication outside of S-phase. |
|---|---|
| المؤلفون: | Johnson, Mark C, Can, Geylani, Santos, Miguel Monteiro, Alexander, Diana, Zegerman, Philip |
| بيانات النشر: | eLife Sciences Publications, Ltd //dx.doi.org/10.7554/elife.63589 Elife |
| سنة النشر: | 2021 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Rad53, S-phase, S. cerevisiae, cell cycle, checkpoints, chromosomes, gene expression, genome stability, replication, Cell Cycle Proteins, Checkpoint Kinase 2, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins |
| الوصف: | Checkpoints maintain the order of cell cycle events during DNA damage or incomplete replication. How the checkpoint response is tailored to different phases of the cell cycle remains poorly understood. The S-phase checkpoint for example results in the slowing of replication, which in budding yeast occurs by Rad53-dependent inhibition of the initiation factors Sld3 and Dbf4. Despite this, we show here that Rad53 phosphorylates both of these substrates throughout the cell cycle at the same sites as in S-phase, suggesting roles for this pathway beyond S-phase. Indeed, we show that Rad53-dependent inhibition of Sld3 and Dbf4 limits re-replication in G2/M, preventing gene amplification. In addition, we show that inhibition of Sld3 and Dbf4 in G1 prevents premature initiation at all origins at the G1/S transition. This study redefines the scope of the 'S-phase checkpoint' with implications for understanding checkpoint function in cancers that lack cell cycle controls. ; Geylani Can GC was supported by a Turkish government grant and a 553 Raymond and Beverley Sackler studentship. Miguel Santos was funded by the BBSRC BB/M011194/1. Diana Alexander was supported by the Department of Biochemistry. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Electronic; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/315809Test |
| DOI: | 10.17863/CAM.62918 |
| الإتاحة: | https://doi.org/10.17863/CAM.62918Test https://www.repository.cam.ac.uk/handle/1810/315809Test |
| حقوق: | Attribution 4.0 International (CC BY) ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.A64889F7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.62918 |
|---|