دورية أكاديمية
Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion.
| العنوان: | Interferon inducible GBPs restrict Burkholderia thailandensis motility induced cell-cell fusion. |
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| المؤلفون: | David E Place, Benoit Briard, Parimal Samir, Rajendra Karki, Anannya Bhattacharya, Clifford S Guy, Jennifer L Peters, Sharon Frase, Peter Vogel, Geoffrey Neale, Masahiro Yamamoto, Thirumala-Devi Kanneganti |
| المصدر: | PLoS Pathogens, Vol 16, Iss 3, p e1008364 (2020) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2020 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
| الوصف: | Innate immunity responds to pathogens by producing alarm signals and activating pathways that make host cells inhospitable for pathogen replication. The intracellular bacterium Burkholderia thailandensis invades the cytosol, hijacks host actin, and induces cell fusion to spread to adjacent cells, forming multinucleated giant cells (MNGCs) which promote bacterial replication. We show that type I interferon (IFN) restricts macrophage MNGC formation during B. thailandensis infection. Guanylate-binding proteins (GBPs) expressed downstream of type I IFN were required to restrict MNGC formation through inhibition of bacterial Arp2/3-dependent actin motility during infection. GTPase activity and the CAAX prenylation domain were required for GBP2 recruitment to B. thailandensis, which restricted bacterial actin polymerization required for MNGC formation. Consistent with the effects in in vitro macrophages, Gbp2-/-, Gbp5-/-, GbpChr3-KO mice were more susceptible to intranasal infection with B. thailandensis than wildtype mice. Our findings reveal that IFN and GBPs play a critical role in restricting cell-cell fusion and bacteria-induced pathology during infection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1553-7366 1553-7374 |
| العلاقة: | https://doi.org/10.1371/journal.ppat.1008364Test; https://doaj.org/toc/1553-7366Test; https://doaj.org/toc/1553-7374Test; https://doaj.org/article/f174a22406714c56bc4c9148d2e0a6cfTest |
| DOI: | 10.1371/journal.ppat.1008364 |
| الإتاحة: | https://doi.org/10.1371/journal.ppat.1008364Test https://doaj.org/article/f174a22406714c56bc4c9148d2e0a6cfTest |
| رقم الانضمام: | edsbas.A592679F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15537366 15537374 |
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| DOI: | 10.1371/journal.ppat.1008364 |