دورية أكاديمية
Treatment with rapamycin can restore regulatory T-cell function in IPEX patients
| العنوان: | Treatment with rapamycin can restore regulatory T-cell function in IPEX patients |
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| المؤلفون: | Passerini L., Barzaghi F., Curto R., Sartirana C., Barera G., Tucci F., Albarello L., Mariani A., Testoni P. A., Bazzigaluppi E., Bosi E., Lampasona V., Neth O., Zama D., Hoenig M., Schulz A., Seidel M. G., Rabbone I., Olek S., Roncarolo M. G., Cicalese M. P., Aiuti A., Bacchetta R. |
| المساهمون: | Passerini L., Barzaghi F., Curto R., Sartirana C., Barera G., Tucci F., Albarello L., Mariani A., Testoni P.A., Bazzigaluppi E., Bosi E., Lampasona V., Neth O., Zama D., Hoenig M., Schulz A., Seidel M.G., Rabbone I., Olek S., Roncarolo M.G., Cicalese M.P., Aiuti A., Bacchetta R. |
| سنة النشر: | 2020 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | autoimmunity, Ebi3, FOXP3, GITR, IPEX, mTOR, rapamycin, regulatory T cell, suppression, Cell Movement, Cells, Cultured, Child, Diabetes Mellitus, Type 1, Diarrhea, Forkhead Transcription Factor, Gene Expression Regulation, Genetic Diseases, X-Linked, Glucocorticoid-Induced TNFR-Related Protein, Human, Immune System Disease, Immune Tolerance, Immunosuppressive Agent, Interleukin, Lymphocyte Activation, Male, Minor Histocompatibility Antigen, Mutation |
| الوصف: | Background: Immune-dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome is a lethal disease caused by mutations in a transcription factor critical for the function of thymus-derived regulatory T (Treg) cells (ie, FOXP3), resulting in impaired Treg function and autoimmunity. At present, hematopoietic stem cell transplantation is the therapy of choice for patients with IPEX syndrome. If not available, multiple immunosuppressive regimens have been used with poor disease-free survival at long-term follow-up. Rapamycin has been shown to suppress peripheral T cells while sparing Treg cells expressing wild-type FOXP3, thereby proving beneficial in the clinical setting of immune dysregulation. However, the mechanisms of immunosuppression selective to Treg cells in patients with IPEX syndrome are unclear. Objective: We sought to determine the cellular and molecular basis of the clinical benefit observed under rapamycin treatment in 6 patients with IPEX syndrome with different FOXP3 mutations. Methods: Phenotype and function of FOXP3-mutated Treg cells from rapamycin-treated patients with IPEX syndrome were tested by flow cytometry and in vitro suppression assays, and the gene expression profile of rapamycin-conditioned Treg cells by droplet-digital PCR. Results: Clinical and histologic improvements in patients correlated with partially restored Treg function, independent of FOXP3 expression or Treg frequency. Expression of TNF-receptor-superfamily-member 18 (TNFRSF18, glucocorticoid-induced TNF-receptor–related) and EBV-induced-3 (EBI3, an IL-35 subunit) in patients’ Treg cells increased during treatment as compared with that of Treg cells from untreated healthy subjects. Furthermore inhibition of glucocorticoid-induced TNF-receptor–related and Ebi3 partially reverted in vitro suppression by in vivo rapamycin-conditioned Treg cells. Conclusions: Rapamycin is able to affect Treg suppressive function via a FOXP3-independent mechanism, thus sustaining the clinical improvement observed in patients with ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31874182; info:eu-repo/semantics/altIdentifier/wos/WOS:000523633400022; volume:145; issue:4; firstpage:1262; lastpage:1271; numberofpages:10; journal:JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY; http://hdl.handle.net/11585/850820Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85078840009 |
| DOI: | 10.1016/j.jaci.2019.11.043 |
| الإتاحة: | https://doi.org/10.1016/j.jaci.2019.11.043Test http://hdl.handle.net/11585/850820Test |
| رقم الانضمام: | edsbas.A591928B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jaci.2019.11.043 |
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