دورية أكاديمية

The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review.

التفاصيل البيبلوغرافية
العنوان: The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review.
المؤلفون: Santisteban-Espejo, Antonio, Bernal-Florindo, Irene, Perez-Requena, Jose, Atienza-Cuevas, Lidia, Moran-Sanchez, Julia, Fernandez-Valle, María Del Carmen, Romero-Garcia, Raquel, Garcia-Rojo, Marcial
سنة النشر: 2022
المجموعة: Sistema Sanitario Público de Andalucía (SSPA): Repositorio
مصطلحات موضوعية: Classic Hodgkin lymphoma, liquid biopsy, next-generation sequencing, standardization
الوصف: Classic Hodgkin lymphoma (cHL) constitutes a B cell-derived neoplasm defined by a scarce tumoral population, termed Hodgkin and Reed-Sternberg (HRS) cells, submerged into a histologically heterogeneous microenvironment. The paucity of HRS cells has historically hampered genetic studies, rendering the identification of the recurrent genetic lesions and molecular pathways deregulated in this lymphoma difficult. The advent of high-throughput sequencing methods such as next-generation sequencing (NGS) could sensibly optimize the identification of the mutational landscape of cHL. However, there is no current consensus either in the design of panels for targeted NGS or in its most relevant clinical applications. In this work, we systematically review the current state of NGS studies of cHL, stressing the need for standardization both in the candidate genes to be analyzed and the bioinformatic pipelines. As different institutions have developed and implemented their own customized NGS-based protocols, to compare and systematically review the major findings of this ongoing research area could be of added value for centers that routinely perform diagnostic, monitoring and genotyping strategies in cHL samples. The results of this systematic review should contribute to the interdepartmental harmonization and achievement of a consensus in the current clinical applications of NGS studies of cHL.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 2075-4418
العلاقة: http://hdl.handle.net/10668/20961Test; PMC9027849; https://www.mdpi.com/2075-4418/12/4/963/pdf?version=1649845552Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027849/pdfTest
DOI: 10.3390/diagnostics12040963
الإتاحة: https://doi.org/10.3390/diagnostics12040963Test
http://hdl.handle.net/10668/20961Test
https://www.mdpi.com/2075-4418/12/4/963/pdf?version=1649845552Test
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9027849/pdfTest
حقوق: Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ ; open access
رقم الانضمام: edsbas.A543087D
قاعدة البيانات: BASE
الوصف
تدمد:20754418
DOI:10.3390/diagnostics12040963