دورية أكاديمية
Genome-wide DNA methylation analysis in an antimigraine-treated preclinical model of cortical spreading depolarization
| العنوان: | Genome-wide DNA methylation analysis in an antimigraine-treated preclinical model of cortical spreading depolarization |
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| المؤلفون: | Vila-Pueyo, Marta, Cuenca León, Ester, Queirós, Ana C., Kulis, Marta, Sintas Vives, Cèlia, Cormand Rifà, Bru, Martín-Subero, José Ignacio, Pozo-Rosich, Patricia, Fernàndez Castillo, Noèlia, Macaya Ruiz, Alfons |
| المصدر: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2023 |
| المجموعة: | Dipòsit Digital de la Universitat de Barcelona |
| مصطلحات موضوعية: | Models animals en la investigació, Epigenètica, Migranya, Animal models in research, Epigenetics, Migraine |
| الوصف: | Background: Cortical spreading depolarization, the cause of migraine aura, is a short-lasting depolarization wave that moves across the brain cortex, transiently suppressing neuronal activity. Prophylactic treatments for migraine, such as topiramate or valproate, reduce the number of cortical spreading depression events in rodents. Objective: To investigate whether cortical spreading depolarization with and without chronic treatment with topiramate or valproate affect the DNA methylation of the cortex. Methods: Sprague-Dawley rats were intraperitoneally injected with saline, topiramate or valproate for four weeks when cortical spreading depolarization were induced and genome-wide DNA methylation was performed in the cortex of six rats per group. Results: The DNA methylation profile of the cortex was significantly modified after cortical spreading depolarization, with and without topiramate or valproate. Interestingly, topiramate reduced by almost 50% the number of differentially methylated regions, whereas valproate increased them by 17%, when comparing to the non-treated group after cortical spreading depolarization induction. The majority of the differentially methylated regions lay within intragenic regions, and the analyses of functional group over-representation retrieved several enriched functions, including functions related to protein processing in the cortical spreading depolarization without treatment group; functions related to metabolic processes in the cortical spreading depolarization with topiramate group; and functions related to synapse and ErbB, MAPK or retrograde endocannabinoid signaling in the cortical spreading depolarization with valproate group. Conclusions: Our results may provide insights into the underlying physiological mechanisms of migraine with aura and emphasize the role of epigenetics in migraine susceptibility. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 12 p.; application/pdf |
| اللغة: | English |
| تدمد: | 0333-1024 |
| العلاقة: | Versió postprint del document publicat a: https://doi.org/10.1177/03331024221146317Test; Cephalalgia, 2023, vol. 43, num. 2, p. 333102422114631; https://doi.org/10.1177/03331024221146317Test; http://hdl.handle.net/2445/197784Test; 724261 |
| الإتاحة: | https://doi.org/10.1177/03331024221146317Test http://hdl.handle.net/2445/197784Test |
| حقوق: | (c) International Headache Society, 2023 ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A46FD69B |
| قاعدة البيانات: | BASE |
| تدمد: | 03331024 |
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