دورية أكاديمية
HaloTag-based reporters for sparse labeling and cell tracking
| العنوان: | HaloTag-based reporters for sparse labeling and cell tracking |
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| المؤلفون: | Couturier, Lydie, Luna, Juan, Mazouni, Khalil, Mestdagh, Claire, Phan, Minh-Son, Corson, Francis, Schweisguth, Francois |
| المساهمون: | Dynamique des Décisions de Développement chez la Drosophile - Dynamics of Developmental Decisions in Drosophila, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Biophysique et Neuroscience Théoriques, Laboratoire de physique de l'ENS - ENS Paris (LPENS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Département de Physique de l'ENS-PSL, École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Département de Physique de l'ENS-PSL, Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL), This work was supported by the Agence Nationale de la Recherche under Grants ANR-10-LABX-0073 and ANR-16-CE13-0003, Fondation pour la Recherche Médicale under Grant FRM-DEQ20180339219., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-16-CE13-0003,ModelPattern,Signalisation et patterning: modéliser, visualiser et manipuler la spécification cellulaire(2016) |
| المصدر: | ISSN: 1933-6934. |
| بيانات النشر: | HAL CCSD Taylor & Francis |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cell tracking, Drosophila, HaloTag, cell fate, cell shape, imaginal disc, live imaging, morphogenesis, single cell analysis, sparse labelling, MESH: Animals, MESH: Cell Tracking, MESH: Single-Cell Analysis, MESH: Drosophila, [SDV.BDD]Life Sciences [q-bio]/Development Biology |
| الوصف: | International audience ; Multiscale analysis of morphogenesis requires to follow and measure in real-time the in vivo behaviour of large numbers of individual cells over long period of time. Despite recent progress, the large-scale automated tracking of cells in developing embryos and tissues remains a challenge. Here we describe a genetic tool for the random and sparse labelling of individual cells in developing Drosophila tissues. This tool is based on the conditional expression of a nuclear HaloTag protein that can be fluorescently labelled upon the irreversible binding of a cell permeable synthetic ligand. While the slow maturation of genetically encoded fluorescent renders the tracking of individual cells difficult in rapidly dividing tissues, nuclear HaloTag proteins allowed for rapid labelling of individual cells in cultured imaginal discs. To study cell shape changes, we also produced an HaloTag version of the actin-bound protein LifeAct. Since sparse labelling facilitates cell tracking, nuclear HaloTag reporters will be useful for the single-cell analysis of fate dynamics in Drosophila tissues cultured ex vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/36323649; hal-04234564; https://hal.science/hal-04234564Test; https://hal.science/hal-04234564/documentTest; https://hal.science/hal-04234564/file/HaloTag-based%20reporters%20for%20sparse%20labeling%20and%20cell%20tracking.pdfTest; PUBMED: 36323649; PUBMEDCENTRAL: PMC9635558 |
| DOI: | 10.1080/19336934.2022.2142460 |
| الإتاحة: | https://doi.org/10.1080/19336934.2022.2142460Test https://hal.science/hal-04234564Test https://hal.science/hal-04234564/documentTest https://hal.science/hal-04234564/file/HaloTag-based%20reporters%20for%20sparse%20labeling%20and%20cell%20tracking.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.A36CD219 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1080/19336934.2022.2142460 |
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