دورية أكاديمية
Increased chromosomal radiosensitivity in asymptomatic carriers of a heterozygous BRCA1 mutation
| العنوان: | Increased chromosomal radiosensitivity in asymptomatic carriers of a heterozygous BRCA1 mutation |
|---|---|
| المؤلفون: | Baert, Annelot, Depuydt, Julie, Van Maerken, Tom, Poppe, Bruce, Malfait, Fransiska, Storm, Katrien, van den Ende, Jenneke, Van Damme, Tim, De Nobele, Sylvia, Perletti, Gianpaolo, De Leeneer, Kim, Claes, Kathleen, Vral, Anne |
| المصدر: | BREAST CANCER RESEARCH ; ISSN: 1465-542X |
| سنة النشر: | 2016 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, nonsense mediated decay, haploinsufficiency, Radiosensitivity indicator, G2 micronucleus assay, Ionizing radiation, G2/M cell-cycle checkpoint, Homologous recombination, BRCA1 mutation, DNA damage repair, BREAST-CANCER RISK, MAMMOGRAPHY X-RAYS, DNA-DAMAGE, IONIZING-RADIATION, HUMAN LYMPHOCYTES, SPLICE SITES, HUMAN-CELLS, REPAIR, INSTABILITY, WOMEN |
| الوصف: | Background: Breast cancer risk increases drastically in individuals carrying a germline BRCA1 mutation. The exposure to ionizing radiation for diagnostic or therapeutic purposes of BRCA1 mutation carriers is counterintuitive, since BRCA1 is active in the DNA damage response pathway. The aim of this study was to investigate whether healthy BRCA1 mutations carriers demonstrate an increased radiosensitivity compared with healthy individuals. Methods: We defined a novel radiosensitivity indicator (RIND) based on two endpoints measured by the G2 micronucleus assay, reflecting defects in DNA repair and G2 arrest capacity after exposure to doses of 2 or 4 Gy. We investigated if a correlation between the RIND score and nonsense-mediated decay (NMD) could be established. Results: We found significantly increased radiosensitivity in the cohort of healthy BRCA1 mutation carriers compared with healthy controls. In addition, our analysis showed a significantly different distribution over the RIND scores (p = 0.034, Fisher’s exact test) for healthy BRCA1 mutation carriers compared with non-carriers: 72 % of mutation carriers showed a radiosensitive phenotype (RIND score 1–4), whereas 72 % of the healthy volunteers showed no radiosensitivity (RIND score 0). Furthermore, 28 % of BRCA1 mutation carriers had a RIND score of 3 or 4 (not observed in control subjects). The radiosensitive phenotype was similar for relatives within several families, but not for unrelated individuals carrying the same mutation. The median RIND score was higher in patients with a mutation leading to a premature termination codon (PTC) located in the central part of the gene than in patients with a germline mutation in the 5′ end of the gene. Conclusions: We show that BRCA1 mutations are associated with a radiosensitive phenotype related to a compromised DNA repair and G2 arrest capacity after exposure to either 2 or 4 Gy. Our study confirms that haploinsufficiency is the mechanism involved in radiosensitivity in patients with a PTC allele, but it ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://biblio.ugent.be/publication/7223141Test; http://hdl.handle.net/1854/LU-7223141Test; http://dx.doi.org/10.1186/s13058-016-0709-1Test; https://biblio.ugent.be/publication/7223141/file/7223429Test |
| DOI: | 10.1186/s13058-016-0709-1 |
| الإتاحة: | https://doi.org/10.1186/s13058-016-0709-1Test https://biblio.ugent.be/publication/7223141Test http://hdl.handle.net/1854/LU-7223141Test https://biblio.ugent.be/publication/7223141/file/7223429Test |
| حقوق: | Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A2D5AC8E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13058-016-0709-1 |
|---|