دورية أكاديمية
Heterologous Expression and Biochemical Characterization of a New Chloroperoxidase Isolated from the Deep-Sea Hydrothermal Vent Black Yeast Hortaea werneckii UBOCC-A-208029
| العنوان: | Heterologous Expression and Biochemical Characterization of a New Chloroperoxidase Isolated from the Deep-Sea Hydrothermal Vent Black Yeast Hortaea werneckii UBOCC-A-208029 |
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| المؤلفون: | Cochereau, Bastien, Le Strat, Yoran, Ji, Qiaolin, Pawtowski, Audrey, Delage, Ludovic, Weill, Amélie, Mazéas, Lisa, Hervé, Cécile, Burgaud, Gaëtan, Gunde-Cimerman, Nina, Pouchus, Yves François, Demont-Caulet, Nathalie, Roullier, Catherine, Meslet-Cladiere, Laurence |
| المساهمون: | Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Institut Des Substances et Organismes de la Mer - UR 2160 (ISOMER), Nantes Université - UFR des Sciences Pharmaceutiques et Biologiques (Nantes Univ - UFR Pharmacie), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Molecular Genetics and Biology of Microorganisms, University of Ljubljana, Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | ISSN: 1436-2228. |
| بيانات النشر: | HAL CCSD Springer Verlag |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Marine fungi, Black yeast, Halogens, Enzymes, Vanadium chloroperoxidase, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] |
| الوصف: | International audience ; The initiation of this study relies on a targeted genome-mining approach to highlight the presence of a putative vanadium-dependent haloperoxidase-encoding gene in the deep-sea hydrothermal vent fungus Hortaea werneckii UBOCC-A-208029. To date, only three fungal vanadium-dependent haloperoxidases have been described, one from the terrestrial species Curvularia inaequalis, one from the fungal plant pathogen Botrytis cinerea , and one from a marine derived isolate identified as Alternaria didymospora . In this study, we describe a new vanadium chloroperoxidase from the black yeast H. werneckii , successfully cloned and overexpressed in a bacterial host, which possesses higher affinity for bromide (K m = 26 µM) than chloride (K m = 237 mM). The enzyme was biochemically characterized, and we have evaluated its potential for biocatalysis by determining its stability and tolerance in organic solvents. We also describe its potential three-dimensional structure by building a model using the AlphaFold 2 artificial intelligence tool. This model shows some conservation of the 3D structure of the active site compared to the vanadium chloroperoxidase from C. inaequalis but it also highlights some differences in the active site entrance and the volume of the active site pocket, underlining its originality. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/37354383; hal-04241644; https://hal.science/hal-04241644Test; https://hal.science/hal-04241644/documentTest; https://hal.science/hal-04241644/file/Cochereau_2023.pdfTest; PUBMED: 37354383; WOS: 001016142600002 |
| DOI: | 10.1007/s10126-023-10222-7 |
| الإتاحة: | https://doi.org/10.1007/s10126-023-10222-7Test https://hal.science/hal-04241644Test https://hal.science/hal-04241644/documentTest https://hal.science/hal-04241644/file/Cochereau_2023.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.A20471A3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s10126-023-10222-7 |
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