دورية أكاديمية
Structurally Informative Tandem Mass Spectrometry of Highly Sulfated Natural and Chemoenzymatically Synthesized Heparin and Heparan Sulfate Glycosaminoglycans
| العنوان: | Structurally Informative Tandem Mass Spectrometry of Highly Sulfated Natural and Chemoenzymatically Synthesized Heparin and Heparan Sulfate Glycosaminoglycans |
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| المؤلفون: | Kailemia, Muchena J., Li, Lingyun, Xu, Yongmei, Liu, Jian, Linhardt, Robert J., Amster, I. Jonathan |
| المصدر: | Molecular & Cellular Proteomics - MCP, 12(4) |
| سنة النشر: | 2013 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Animals, Heparitin Sulfate, Phosphoadenosine Phosphosulfate, Heparin, Tandem Mass Spectrometry, Carbohydrate Sequence, Sulfotransferases, Carbohydrate Conformation, Sulfuric Acid Esters, Oligosaccharides, Molecular Sequence Data, Sus scrofa, Biocatalysis, Spectrometry, Mass, Electrospray Ionization |
| الوصف: | The highly sulfated glycosaminoglycan oligosaccharides derived from heparin and heparan sulfate have been a highly intractable class of molecules to analyze by tandem mass spectrometry. Under the many methods of ion activation, this class of molecules generally exhibits SO3 loss as the most significant fragmentation pathway, interfering with the assignment of the location of sulfo groups in glycosaminoglycan chains. We report here a method that stabilizes sulfo groups and facilitates the complete structural analysis of densely sulfated (two or more sulfo groups per disaccharide repeat unit) heparin and heparan sulfate oligomers. This is achieved by complete removal of all ionizable protons, either by charging during electrospray ionization or by Na+/H+ exchange. The addition of millimolar levels of NaOH to the sample solution facilitates the production of precursor ions that meet this criterion. This approach is found to work for a variety of heparin sulfate oligosaccharides derived from natural sources or produced by chemoenzymatic synthesis, with up to 12 saccharide subunits and up to 11 sulfo groups. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/8hx1-d578Test; https://cdr.lib.unc.edu/downloads/0c483s07g?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/0c483s07gTest |
| DOI: | 10.17615/8hx1-d578 |
| الإتاحة: | https://doi.org/10.17615/8hx1-d578Test https://cdr.lib.unc.edu/downloads/0c483s07g?file=thumbnailTest https://cdr.lib.unc.edu/downloads/0c483s07gTest |
| حقوق: | http://rightsstatements.org/vocab/InC/1.0Test/ |
| رقم الانضمام: | edsbas.A1F4E8C2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/8hx1-d578 |
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