التفاصيل البيبلوغرافية
| العنوان: |
Preparation of screening assays for ADP-ribosyl readers and erasers using the GAP-tag as a binding probe |
| المؤلفون: |
Sowa, S. T. (Sven T.), Galera-Prat, A. (Albert), Wazir, S. (Sarah), Alanen, H. I. (Heli I.), Maksimainen, M. M. (Mirko M.), Lehtiö, L. (Lari) |
| بيانات النشر: |
Elsevier |
| سنة النشر: |
2022 |
| المجموعة: |
Jultika - University of Oulu repository / Oulun yliopiston julkaisuarkisto |
| مصطلحات موضوعية: |
High Throughput Screening, Molecular/Chemical Probes, Protein Biochemistry, Protein expression and purification, Signal Transduction |
| الوصف: |
Here, we describe a protocol to set up a screening assay for ADP-ribosyl binding proteins including proteins that possess O-glycosidase or N-glycosidase activities. The FRET-based assay measures the interaction of any ADP-ribosyl binding protein fused to CFP with a cysteine-ADP-ribosylated GAP-tag fused to YFP. Recombinant PtxS1 and PARP2 are used to mono-ADP-ribosylate and poly-ADP-ribosylate the GAP-tag. The protocol does not require specialized compounds or substrates, making it accessible and easy to adapt in any laboratory or for other proteins of interest. For complete details on the use and execution of this profile, please refer to Sowa et al. (2021). |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| الإتاحة: |
http://urn.fi/urn:nbn:fi-fe2022051335061Test |
| حقوق: |
info:eu-repo/semantics/openAccess ; © 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0Test/). ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: |
edsbas.A1D3D4C9 |
| قاعدة البيانات: |
BASE |
