التفاصيل البيبلوغرافية
| العنوان: |
HIV‐1 triggers apoptosis in primary osteoblasts and HOBIT cells through TNFα activation |
| المؤلفون: |
Gibellini, Davide, De Crignis, Elisa, Ponti, Cristina, Cimatti, Laura, Borderi, Marco, Tschon, Matilde, Giardino, Roberto, Re, Maria Carla |
| المصدر: |
Journal of Medical Virology ; volume 80, issue 9, page 1507-1514 ; ISSN 0146-6615 1096-9071 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2008 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Several HIV‐1 infected patients show bone loss and osteopenia/osteoporosis during the course of disease. The mechanisms underlying this degenerative process are largely unsettled and it has not been determined yet whether bone dysfunction is linked to HIV‐1‐mediated direct and/or indirect effects on osteoblasts/osteoclasts cross‐talk regulation. This study investigated the effects of HIV‐1 IIIb and HIV‐1 ADA strains on osteoblasts using the osteoblast‐derived cell line (HOBIT) and primary human osteoblasts as cellular models. The challenge of these cell cultures by both HIV‐1 strains triggered a significant apoptosis activation unrelated to viral infection, since proviral HIV‐1 DNA and supernatant HIV‐1 RNA were not detected by real time PCR or b‐DNA assays respectively. Under the experimental conditions, even heat‐inactivated HIV‐1 or cross‐linked recombinant gp120 treatment of HOBIT and osteoblasts induced programmed cell death, suggesting that apoptosis is regulated by the interaction between HIV‐1 gp120 and cell membrane. The analysis of cell culture supernatants showed a significant up‐regulation of TNFα, a pleiotropic protein considered an apoptosis inducer in the osteoblast model. In fact, pretreatment of HOBIT and osteoblast cell cultures with anti‐TNFα polyclonal antibody tackled effectively HIV‐1 related induction of cell apoptosis. As a whole, these results indicate that HIV‐1 may impair bone mass structure homeostasis by TNFα regulated osteoblast apoptosis. J. Med. Virol. 80:1507–1514, 2008. © 2008 Wiley‐Liss, Inc. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/jmv.21266 |
| الإتاحة: |
https://doi.org/10.1002/jmv.21266Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.A1C5D3B2 |
| قاعدة البيانات: |
BASE |
