دورية أكاديمية
A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient
| العنوان: | A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient |
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| المؤلفون: | Bitounis, Dimitrios, Barnier, Vincent, Guibert, Cyril, Pourchez, Jérémie, Forest, Valérie, Boudard, Delphine, Hochepied, Jean-François, Chelle, Pierre, Vergnon, Jean-Michel, Cottier, Michèle |
| المساهمون: | Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Georges Friedel (LGF-ENSMSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Service de Pneumologie et Oncologie thoracique CHU Saint-Etienne, Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon, F-69000 Lyon, France, Service de Cytologie - Histologie Rénale - Embryologie CHU Saint-Etienne (UF 6725 - UF 6726), Centre des Matériaux (CDM), Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 2040-3364. |
| بيانات النشر: | HAL CCSD Royal Society of Chemistry |
| سنة النشر: | 2018 |
| المجموعة: | Université Jean Monnet – Saint-Etienne: HAL |
| مصطلحات موضوعية: | [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], [SDV]Life Sciences [q-bio], [SDV.IB]Life Sciences [q-bio]/Bioengineering |
| الوصف: | International audience ; Bronchoalveolar lavage (BAL) is a diagnostic procedure which samples the cellular and non-cellular components of the pulmonary epithelial surface. The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspected to impose quantitative and qualitative bias on the studied particle load. This study presents a simple method for the near-lossless extraction of citrate-capped gold nanoparticles from human BAL fluids at sub-ppm levels which enables their quantitation and surface characterization. This procedure was modeled according to fundamental principles of particle sedimentation and liquid–liquid interdiffusion and was evaluated by a battery of analytical techniques. The extraction yield of gold nanoparticles ranged from 61 to 86%, with a quantitation limit at 0.5 μg ml−1, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles’ biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01806118; https://minesparis-psl.hal.science/hal-01806118Test; https://minesparis-psl.hal.science/hal-01806118/documentTest; https://minesparis-psl.hal.science/hal-01806118/file/HAL_Bitounis%20et%20al_2018_Nanoscale.pdfTest |
| DOI: | 10.1039/C7NR04484D |
| الإتاحة: | https://doi.org/10.1039/C7NR04484DTest https://minesparis-psl.hal.science/hal-01806118Test https://minesparis-psl.hal.science/hal-01806118/documentTest https://minesparis-psl.hal.science/hal-01806118/file/HAL_Bitounis%20et%20al_2018_Nanoscale.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.A18107FD |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/C7NR04484D |
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