دورية أكاديمية
Molecular characterization, protein–protein interaction network, and evolution of four glutathione peroxidases from Tetrahymena thermophila
| العنوان: | Molecular characterization, protein–protein interaction network, and evolution of four glutathione peroxidases from Tetrahymena thermophila |
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| المؤلفون: | Ferro D., Bakiu R., Pucciarelli S., Miceli C., Vallesi A., Irato P., Santovito G. |
| المساهمون: | Ferro, D., Bakiu, R., Pucciarelli, S., Miceli, C., Vallesi, A., Irato, P., Santovito, G. |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2020 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: | Antioxidant system, Ciliate protist, Copper, Free-radical, Glutathione peroxidases gene, GPx, Metal, Protein–protein interaction network, Reactive oxygen specie, ROS |
| الوصف: | Glutathione peroxidases (GPxs) form a broad family of antioxidant proteins essential for maintaining redox homeostasis in eukaryotic cells. In this study, we used an integrative approach that combines bioinformatics, molecular biology, and biochemistry to investigate the role of GPxs in reactive oxygen species detoxification in the unicellular eukaryotic model organism Tetrahymena thermophila. Both phylogenetic and mechanistic empirical model analyses provided indications about the evolutionary relationships among the GPXs of Tetrahymena and the orthologous enzymes of phylogenetically related species. In-silico gene characterization and text mining were used to predict the functional relationships between GPxs and other physiologically-relevant processes. The GPx genes contain conserved transcriptional regulatory elements in the promoter region, which suggest that transcription is under tight control of specialized signaling pathways. The bioinformatic findings were next experimentally validated by studying the time course of gene transcription and enzymatic activity after copper (Cu) exposure. Results emphasize the role of GPxs in the detoxification pathways that, by complex regulation of GPx gene expression, enable Tethraymena to survive in high Cu concentrations and the associated redox environment. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000584197800001; volume:9; issue:10; firstpage:1; lastpage:15; numberofpages:15; journal:ANTIOXIDANTS; http://hdl.handle.net/11577/3354044Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85092037034 |
| DOI: | 10.3390/antiox9100949 |
| الإتاحة: | https://doi.org/10.3390/antiox9100949Test http://hdl.handle.net/11577/3354044Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.A104D22B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/antiox9100949 |
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