دورية أكاديمية
Concordant and discordant DNA methylation signatures of aging in human blood and brain
| العنوان: | Concordant and discordant DNA methylation signatures of aging in human blood and brain |
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| المؤلفون: | Farré, Pau, Jones, Meaghan J, Meaney, Michael J, Emberly, Eldon, Turecki, Gustavo, Kobor, Michael S |
| المساهمون: | Child and Family Research Institute |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2015 |
| المجموعة: | University of British Columbia: cIRcle - UBC's Information Repository |
| مصطلحات موضوعية: | DNA methylation, Principal component analysis, Aging, Brain, Blood, Epigenetics |
| الوصف: | Background. DNA methylation is an epigenetic mark that balances plasticity with stability. While DNA methylation exhibits tissue specificity, it can also vary with age and potentially environmental exposures. In studies of DNA methylation, samples from specific tissues, especially brain, are frequently limited and so surrogate tissues are often used. As yet, we do not fully understand how DNA methylation profiles of these surrogate tissues relate to the profiles of the central tissue of interest. Results We have adapted principal component analysis to analyze data from the Illumina 450K Human Methylation array using a set of 17 individuals with 3 brain regions and whole blood. All of the top five principal components in our analysis were associated with a variable of interest: principal component 1 (PC1) differentiated brain from blood, PCs 2 and 3 were representative of tissue composition within brain and blood, respectively, and PCs 4 and 5 were associated with age of the individual (PC4 in brain and PC5 in both brain and blood). We validated our age-related PCs in four independent sample sets, including additional brain and blood samples and liver and buccal cells. Gene ontology analysis of all five PCs showed enrichment for processes that inform on the functions of each PC. Conclusions Principal component analysis (PCA) allows simultaneous and independent analysis of tissue composition and other phenotypes of interest. We discovered an epigenetic signature of age that is not associated with cell type composition and required no correction for cellular heterogeneity. ; Medical Genetics, Department of ; Medicine, Faculty of ; Molecular Medicine and Therapeutics, Centre for ; Non UBC ; Reviewed ; Faculty |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Epigenetics & Chromatin. 2015 May 09;8(1):19; http://hdl.handle.net/2429/54766Test |
| DOI: | 10.1186/s13072-015-0011-y |
| الإتاحة: | https://doi.org/10.1186/s13072-015-0011-yTest http://hdl.handle.net/2429/54766Test |
| حقوق: | Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ ; Farré et al.; licensee BioMed Central. |
| رقم الانضمام: | edsbas.A0635C83 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13072-015-0011-y |
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