دورية أكاديمية
Extent, pattern, and prognostic value of MGMT promotor methylation: does it differ between glioblastoma and IDH-wildtype/TERT-mutated astrocytoma?
| العنوان: | Extent, pattern, and prognostic value of MGMT promotor methylation: does it differ between glioblastoma and IDH-wildtype/TERT-mutated astrocytoma? |
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| المؤلفون: | Teske, Nico, Karschnia, Philipp, Weller, Jonathan, Siller, Sebastian, Dorostkar, Mario M., Herms, Jochen, von Baumgarten, Louisa, Tonn, Joerg Christian, Thon, Niklas |
| المصدر: | http://lobid.org/resources/99370674912706441Test#!, 156(2):317-327. |
| سنة النشر: | 2021 |
| المجموعة: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| مصطلحات موضوعية: | Glioma, Telomerase/genetics [MeSH], Mutation [MeSH], WHO CNS 2021, Tumor Suppressor Proteins/genetics [MeSH], Brain Neoplasms/genetics [MeSH], Humans [MeSH], Astrocytoma/pathology [MeSH], DNA Repair Enzymes/genetics [MeSH], Glioblastoma/pathology [MeSH], DNA Modification Methylases/genetics [MeSH], TERT, DNA Methylation [MeSH], IDH wildtype, Prognosis [MeSH], cIMPACT, Astrocytoma/genetics [MeSH], Clinical Study, Glioblastoma/genetics [MeSH], Isocitrate Dehydrogenase/genetics [MeSH], Brain Neoplasms/pathology [MeSH], Promoter Regions, Genetic [MeSH] |
| الوصف: | Introduction!#!The cIMPACT-NOW update 6 first introduced glioblastoma diagnosis based on the combination of IDH-wildtype (IDHwt) status and TERT promotor mutation (pTERTmut). In glioblastoma as defined by histopathology according to the WHO 2016 classification, MGMT promotor status is associated with outcome. Whether this is also true in glioblastoma defined by molecular markers is yet unclear.!##!Methods!#!We searched the institutional database for patients with: (1) glioblastoma defined by histopathology; and (2) IDHwt astrocytoma with pTERTmut. MGMT promotor methylation was analysed using methylation-specific PCR and Sanger sequencing of CpG sites within the MGMT promotor region.!##!Results!#!We identified 224 patients with glioblastoma diagnosed based on histopathology, and 54 patients with IDHwt astrocytoma with pTERTmut (19 astrocytomas WHO grade II and 38 astrocytomas WHO grade III). There was no difference in the number of MGMT methylated tumors between the two cohorts as determined per PCR, and also neither the number nor the pattern of methylated CpG sites differed as determined per Sanger sequencing. Progression-free (PFS) and overall survival (OS) was similar between the two cohorts when treated with radio- or chemotherapy. In both cohorts, higher numbers of methylated CpG sites were associated with favourable outcome.!##!Conclusions!#!Extent and pattern of methylated CpG sites are similar in glioblastoma and IDHwt astrocytoma with pTERTmut. In both tumor entities, higher numbers of methylated CpG sites appear associated with more favourable outcome. Evaluation in larger prospective cohorts is warranted. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://repository.publisso.de/resource/frl:6445973Test; https://doi.org/10.1007/s11060-021-03912-6Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816375Test/ |
| DOI: | 10.1007/s11060-021-03912-6 |
| الإتاحة: | https://doi.org/10.1007/s11060-021-03912-6Test https://repository.publisso.de/resource/frl:6445973Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816375Test/ |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.A0064FE1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s11060-021-03912-6 |
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