دورية أكاديمية
New Strategy of Reducing Biofilm Forming Bacteria in Oral Cavity by Bismuth Nanoparticles
| العنوان: | New Strategy of Reducing Biofilm Forming Bacteria in Oral Cavity by Bismuth Nanoparticles |
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| المؤلفون: | Sahar Rostamifar, Azita Azad, Ali Bazrafkan, Farzan Modaresi, Shekoufeh Atashpour, Zahra Kargar Jahromi |
| المصدر: | BioMed Research International, Vol 2021 (2021) |
| بيانات النشر: | Hindawi Limited |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine |
| الوصف: | Objective. Enterococcus faecalis and Streptococcus salivarius are the most important species in dental decay and producing biofilm. Treatment with chlorhexidine 2% mouthwash for 7 days is the best way to eliminate these bacteria. However, due to the ability of these bacteria to survive in harsh environments, increasing emergence of bacterial resistance against available antibiotics, and favorable properties of nanoparticles including broad spectrum antimicrobial activity and lower toxicity, we decided to evaluate reducing biofilm forming bacteria in oral cavity by bismuth nanoparticles. Materials and Methods. This was a cross-sectional study of 40 samples isolated from the patients visiting dental clinics in Shiraz in 2019. Samples, which showed growth, were cultured on blood agar plates and incubated for the PCR procedure. Nanoparticle powder was dissolved in high-purity water, and the final concentration of bismuth nanoparticles (BiNPs) was measured with a spectrophotometer. Minimum inhibitory concentration (MIC) of BiNPs against E. faecalis and S. salivarius was determined by the microbroth dilution method according to methods for antimicrobial susceptibility tests. Also, bactericidal assays were conducted in a Mueller-Hinton broth medium and reported as the concentration of BiNPs that reduced the viable bacterial count by 99.9%. Statistical analysis was carried out using SPSS 21 and one-way analysis of variance, and P values less than 0.05 were considered significant. Results. MICs of BiNP suspension against Streptococcus salivarius and Enterococcus faecalis were 2.5 and 5 μg/ml, respectively. Minimum bactericidal concentrations (MBC) of BiNP suspension against Streptococcus salivarius and Enterococcus faecalis were 5 and 10 μg/ml, respectively. Antibacterial activity of BiNPs was compared with chlorhexidine 2%. MICs of BiNPs against Streptococcus salivarius and Enterococcus faecalis were one-twentieth less than those of chlorhexidine. MBC of BiNPs against both pathogens was one-tenth less than those of ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2314-6133 2314-6141 |
| العلاقة: | http://dx.doi.org/10.1155/2021/6695692Test; https://doaj.org/toc/2314-6133Test; https://doaj.org/toc/2314-6141Test; https://doaj.org/article/d9b45e1a901e4ef09c0d71cbd53d9bc5Test |
| DOI: | 10.1155/2021/6695692 |
| الإتاحة: | https://doi.org/10.1155/2021/6695692Test https://doaj.org/article/d9b45e1a901e4ef09c0d71cbd53d9bc5Test |
| رقم الانضمام: | edsbas.9DF2B340 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23146133 23146141 |
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| DOI: | 10.1155/2021/6695692 |