دورية أكاديمية
Genetic transformation and siRNA-Mediated gene silencing for aphid resistance in tomato
| العنوان: | Genetic transformation and siRNA-Mediated gene silencing for aphid resistance in tomato |
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| المؤلفون: | Faisal, Mohammad, Abdel-Salam, Eslam M., Alatar, Abdulrahman A., Saquib, Quaiser, Alwathnani, Hend A., Canto, Tomás |
| المساهمون: | King Abdulaziz City for Science and Technology |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Agrobacterium tumefaciens, Genetic transformation, Horticulture crop, Northern blotting, RNAi |
| الوصف: | © The Author(s). ; We explored the ability of RNA interference (RNAi) to silence the Acetylcholinesterase 1 (Ace 1) gene in aphid Myzus persicae and developed transgenic tomato plants resistant to aphid infestation. Three plasmid constructs, T-449: a single Ace 1 fragment (forward orientation), T-452: two Ace 1 fragments (reverse and forward orientations), and T455: a single inverted Ace 1 fragment, were developed and transformed into two tomato cultivars, Jamila and Tomaland. PCR, northern blotting, and small interfering RNAs (siRNA) analysis were performed to validate the success of Agrobacterium-mediated transformation. The efficiency of transformation was highest for the T-452 construct. In vivo effects of the transformed constructs were confirmed in feeding experiments, and there was significant downregulation of the Ace 1 gene. In addition, an aphid challenge assay was conducted to investigate the siRNA-mediated silencing of the target gene (Ace 1) in the inhibition of fecundity in M. persicae. We found that the plants that were transformed with the T-452 vector had 37.5% and 26.4% lower fecundity at 27 °C in the Jamila and Tomaland, respectively. Our results strongly indicated that the plant-mediated silencing of aphid-RNA might be a robust and effective approach for developing pest and disease resistant in plants ; This project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdul Aziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number 12-BIO2919-02. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 2073-4395 |
| العلاقة: | Publisher’s version; https://doi.org/10.3390/agronomy9120893Test; Sí; Agronomy 9(12): 893 (2019); http://hdl.handle.net/10261/197145Test; http://dx.doi.org/10.13039/501100004919Test |
| DOI: | 10.3390/agronomy9120893 |
| DOI: | 10.13039/501100004919 |
| الإتاحة: | https://doi.org/10.3390/agronomy9120893Test https://doi.org/10.13039/501100004919Test http://hdl.handle.net/10261/197145Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.9D276E0F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20734395 |
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| DOI: | 10.3390/agronomy9120893 |