دورية أكاديمية
Performance of microbiological tests for tuberculosis diagnostic according to the type of respiratory specimen: A 10-year retrospective study.
| العنوان: | Performance of microbiological tests for tuberculosis diagnostic according to the type of respiratory specimen: A 10-year retrospective study. |
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| المؤلفون: | Boldi, M.O., Denis-Lessard, J., Neziri, R., Brouillet, R., von-Garnier, C., Chavez, V., Mazza-Stalder, J., Jaton, K., Greub, G., Opota, O. |
| المصدر: | Frontiers in cellular and infection microbiology, vol. 13, pp. 1131241 |
| سنة النشر: | 2023 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Humans, Mycobacterium tuberculosis/genetics, Retrospective Studies, Tuberculosis, Pulmonary/diagnosis, Pulmonary/microbiology, Sensitivity and Specificity, Bronchoalveolar Lavage Fluid/microbiology, Sputum/microbiology, PCR, acid-fast bacilli (AFB), bronchial aspirate, bronchoalveolar lavage (BAL), induced-sputum, mycobacterial culture, sputum |
| الوصف: | The microbial diagnosis of tuberculosis (TB) remains challenging and relies on multiple microbiological tests performed on different clinical specimens. Polymerase chain reactions (PCRs), introduced in the last decades has had a significant impact on the diagnosis of TB. However, questions remain about the use of PCRs in combination with conventional tests for TB, namely microscopy and culture. We aimed to determine the performance of microscopy, culture and PCR for the diagnosis of pulmonary tuberculosis according to the type of clinical specimen in order to improve the diagnostic yield and to avoid unnecessary, time and labor-intensive tests. We conducted a retrospective study (2008-2018) on analysis (34'429 specimens, 14'358 patients) performed in our diagnostic laboratory located in the Lausanne University Hospital to compare the performance of microbiological tests on sputum, induced sputum, bronchial aspirate and bronchoalveolar lavage (BAL). We analysed the performance using a classical "per specimen" approach and a "per patient" approach for paired specimens collected from the same patient. The overall sensitivities of microscopy, PCR and culture were 0.523 (0.489, 0.557), 0.798 (0.755, 0.836) and 0.988 (0.978, 0.994) and the specificity were 0.994 (0.993, 0.995), 1 (0.999, 1) and 1 (1, 1). Microscopy displayed no significant differences in sensitivity according to the type of sample. The sensitivities of PCR for sputum, induced sputum, bronchial aspirate and BAL were, 0.821 (0.762, 0.871), 0.643 (0.480, 0.784), 0.837 (0.748, 0.904) and 0.759 (0.624, 0.865) respectively and the sensitivity of culture were, 0.993 (0.981, 0.998), 0.980 (0.931, 0.998), 0.965 (0.919, 0.988), and 1 (0.961, 1) respectively. Pairwise comparison of specimens collected from the same patient reported a significantly higher sensitivity of PCR on bronchial aspirate over BAL (p < 0.001) and sputum (p < 0.05) and a significantly higher sensitivity of culture on bronchial aspirate over BAL (p < 0.0001). PCR displayed a higher ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2235-2988 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/36936773; info:eu-repo/semantics/altIdentifier/eissn/2235-2988; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_F4A71366B41B8; https://serval.unil.ch/notice/serval:BIB_F4A71366B41BTest; urn:issn:2235-2988; https://serval.unil.ch/resource/serval:BIB_F4A71366B41B.P001/REF.pdfTest; http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_F4A71366B41B8Test |
| DOI: | 10.3389/fcimb.2023.1131241 |
| الإتاحة: | https://doi.org/10.3389/fcimb.2023.1131241Test https://serval.unil.ch/notice/serval:BIB_F4A71366B41BTest https://serval.unil.ch/resource/serval:BIB_F4A71366B41B.P001/REF.pdfTest http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_F4A71366B41B8Test |
| حقوق: | info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.9D1A8477 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22352988 |
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| DOI: | 10.3389/fcimb.2023.1131241 |