التفاصيل البيبلوغرافية
| العنوان: |
The effect of dietary protein levels on the expression of genes coding for four selected protein translation initiation factors in muscle tissue of Wujin pig |
| المؤلفون: |
Liu, L. Y., Wang, J., Huang, Y., Pan, H. B., Zhang, X., Huang, Z. X., Zhao, S. M., Gao, S. Z. |
| المساهمون: |
Natural Science Foundation of Yunnan Province, National Key Foundation Research Development Project of China |
| المصدر: |
Journal of Animal Physiology and Animal Nutrition ; volume 98, issue 2, page 310-317 ; ISSN 0931-2439 1439-0396 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2013 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Summary The objective of this study was to investigate the regulatory mechanism underlying the increased muscle protein accumulation in pigs while were fed a high protein diet. The eukaryotic initiation factors (e IF s) have been reported to involve in muscle protein synthesis. We investigated the m RNA and protein expression levels of e IF 2 B 1, 4 A 1, 4 B and 4 E in W ujin pigs fed either a high protein ( HP : 18%) or a low protein ( LP : 14%) diet at 30, 60 or 100 kg body weight, based on real‐time PCR and western blotting analyses. Our results indicated that the expression levels of e IF 2 B 1 m RNA and protein were increased by HP diet at all body weight. The HP diet showed higher m RNA and protein levels of e IF 4 B gene at 60 and 100 kg. The protein expression of e IF 4 E phosphorylation was increased by HP diet only at 30 kg. These data suggested that the HP diet promoted porcine muscle protein accumulation mainly by up‐regulating e IF 2 B 1, 4 B and 4 E rather than 4 A 1 expression along the growth stages. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1111/jpn.12081 |
| الإتاحة: |
https://doi.org/10.1111/jpn.12081Test |
| حقوق: |
http://onlinelibrary.wiley.com/termsAndConditions#vorTest |
| رقم الانضمام: |
edsbas.9BED6F77 |
| قاعدة البيانات: |
BASE |
