مؤتمر
Effect of Growth Hormone Secretagogue Receptor Deletion on Growth, Pulsatile Growth Hormone Secretion, and Meal Pattern in Male and Female Mice
| العنوان: | Effect of Growth Hormone Secretagogue Receptor Deletion on Growth, Pulsatile Growth Hormone Secretion, and Meal Pattern in Male and Female Mice |
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| المؤلفون: | Labarthe, Alexandra, Zizzari, Philippe, Fiquet, Oriane, Lebrun, Nicolas, Veldhuis, Johannes, Roelfsema, Ferdinand, Chauveau, Christophe, Bohlooly-Y, Mohammad, Epelbaum, Jacques, Tolle, Virginie |
| المساهمون: | Institut de psychiatrie et neurosciences de Paris IPNP - U1266 Inserm, Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale U1215 Inserm - UB, Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 MABLab (ex-pmoi), Université du Littoral Côte d'Opale ULCO, Mécanismes Adaptatifs et Evolution MECADEV |
| بيانات النشر: | Karger |
| سنة النشر: | 2022 |
| المجموعة: | LillOA (Lille Open Archive - Université de Lille) |
| مصطلحات موضوعية: | Ghrelin, Growth hormone, Growth hormone secretagogue receptor signaling, Meal pattern, Sexual dimorphism |
| الوصف: | Introduction: While the vast majority of research investigating the role of ghrelin or its receptor, GHS-R1a, in growth, feeding, and metabolism has been conducted in male rodents, very little is known about sex differences in this system. Furthermore, the role of GHS-R1a signaling in the control of pulsatile GH secretion and its link with growth or metabolic parameters has never been characterized.Methods: We assessed the sex-specific contribution of GHS-R1a signaling in the activity of the GH/IGF-1 axis, metabolic parameters, and feeding behavior in adolescent (5-6 weeks old) or adult (10-19 weeks old) GHS-R KO (Ghsr-/-) and WT (Ghsr+/+) male and female mice.Results: Adult Ghsr-/- male and female mice displayed deficits in weight and linear growth that were correlated with reduced GH pituitary contents in males only. GHS-R1a deletion was associated with reduced meal frequency and increased meal intervals, as well as reduced hypothalamic GHRH and NPY mRNA in males, not females. In adult, GH release from Ghsr-/- mice pituitary explants ex vivo was reduced independently of the sex. However, in vivo pulsatile GH secretion decreased in adult but not adolescent Ghsr-/- females, while in males, GHS-R1a deletion was associated with reduction in pulsatile GH secretion during adolescence exclusively. In males, linear growth did not correlate with pulsatile GH secretion, but rather with ApEn, a measure that reflects irregularity of the rhythmic secretion. Fat mass, plasma leptin concentrations, or ambulatory activity did not predict differences in GH secretion.Discussion/conclusion: These results point to a sex-dependent dimorphic effect of GHS-R1a signaling to modulate pulsatile GH secretion and meal pattern in mice with different compensatory mechanisms occurring in the hypothalamus of adult males and females after GHS-R1a deletion. Altogether, we show that GHS-R1a signaling plays a more critical role in the regulation of pulsatile GH secretion during adolescence in males and adulthood in females. ; 112 ; 3 |
| نوع الوثيقة: | conference object |
| وصف الملف: | application/octet-stream |
| اللغة: | English |
| العلاقة: | Neuroendocrinology; http://hdl.handle.net/20.500.12210/93779Test |
| الإتاحة: | https://doi.org/20.500.12210/93779Test https://hdl.handle.net/20.500.12210/93779Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.9B93A6B9 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |