التفاصيل البيبلوغرافية
| العنوان: |
Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.) |
| المؤلفون: |
Yang, Qian, Deng, Wei, Li, Xuefeng, Yu, Qin, Bai, Lianyang, Zheng, Mingqi |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2016 |
| المجموعة: |
BioMed Central |
| مصطلحات موضوعية: |
Acetolactate synthase, Cytochrome P450, Flixweed, Tribenuron-methyl, Target-site based resistance, Non-target-site based resistance, Metabolic resistance, RNA-Seq |
| الوصف: |
Background Flixweed ( Descurainia sophia L.) is a troublesome and widespread broadleaf weed in winter fields in China, and has evolved high level resistance to acetolactate synthase (ALS)-inhibiting sulfonylurea herbicide tribenuron-methyl. Results We identified a resistant flixweed population (N11) exhibiting 116.3-fold resistance to tribenuron-methyl relative to the susceptible population (SD8). Target-site ALS gene mutation Pro-197-Thr was identified in resistant plants. Moreover, the resistance can be reversed to 28.7-fold by the cytochrome P450 inhibitor malathion. The RNA-Sequencing was employed to identify candidate genes involved in non-target-site metabolic resistance in this population. Total 26 differentially expressed contigs were identified and eight of them (four P450s, one ABC transporter, three glycosyltransferase) verified by qRT-PCR. Consistent over-expression of the two contigs homology to CYP96A13 and ABCC1 transporter, respectively, were further qRT-PCR validated using additional plants from the resistant and susceptible populations. Conclusions Tribenuron-methyl resistance in flixweed is controlled by target-site ALS mutation and non-target-site based mechanisms. Two genes, CYP96A13 and ABCC1 transporter, could play an important role in metabolic resistance to tribenuron-methyl in the resistant flixweed population and justify further functional studies. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
http://www.biomedcentral.com/1471-2164/17/551Test |
| الإتاحة: |
http://www.biomedcentral.com/1471-2164/17/551Test |
| حقوق: |
Copyright 2016 The Author(s). |
| رقم الانضمام: |
edsbas.9B0E62B6 |
| قاعدة البيانات: |
BASE |
